Application | Comment | Organism |
---|---|---|
pharmacology | analysis of uptake of the antibiotic albomycin into cells of Streptococcus pneumoniae | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
fhuD derivative, expressed in Escherichia coli DH5-alpha and BL21-DE3 and SIP401, different plasmids used | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | insertion duplication mutants and deletion mutants generated in the fhuD, fhuB, and fhuG genes, complementation studies performed, mutants in the fhuD, fhuB and fhuG genes shown to be transport inactive and resistant to the antibiotics albomycin and salmycin | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | cross-inhibition of ferric hydroxamates to the antibiotics albomycin and salmycin determined | Streptococcus pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | FhuD protein | Streptococcus pneumoniae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + ferrichrome/out | Streptococcus pneumoniae | - |
ADP + phosphate + ferrichrome/in | - |
? | |
ATP + H2O + ferrioxamine B/out | Streptococcus pneumoniae | - |
ADP + phosphate + ferrioxamine B/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
R6 strain | - |
Purification (Comment) | Organism |
---|---|
recombinant FhuD protein, gel filtration | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ABC transporter system characterized, albomycin and salmycin sensitivity assays described, ferrichrome and ferrioxamine B transport assays applied, active transport of albomycin by the ABC transporter shown to mediate the high antibiotic efficacy of Streptococcus pneumoniae | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + albomycin/out | - |
Streptococcus pneumoniae | ADP + phosphate + albomycin/in | - |
? | |
ATP + H2O + ferrichrome/out | - |
Streptococcus pneumoniae | ADP + phosphate + ferrichrome/in | - |
? | |
ATP + H2O + ferrioxamine B/out | - |
Streptococcus pneumoniae | ADP + phosphate + ferrioxamine B/in | - |
? | |
ATP + H2O + salmycin/out | - |
Streptococcus pneumoniae | ADP + phosphate + salmycin/in | - |
? |