Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.16 extracted from

  • Pramanik, A.; Braun, V.
    Albomycin uptake via a ferric hydroxamate transport system of Streptococcus pneumoniae R6 (2006), J. Bacteriol., 188, 3878-3886.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
pharmacology analysis of uptake of the antibiotic albomycin into cells of Streptococcus pneumoniae Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
fhuD derivative, expressed in Escherichia coli DH5-alpha and BL21-DE3 and SIP401, different plasmids used Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information insertion duplication mutants and deletion mutants generated in the fhuD, fhuB, and fhuG genes, complementation studies performed, mutants in the fhuD, fhuB and fhuG genes shown to be transport inactive and resistant to the antibiotics albomycin and salmycin Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
additional information cross-inhibition of ferric hydroxamates to the antibiotics albomycin and salmycin determined Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm FhuD protein Streptococcus pneumoniae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + ferrichrome/out Streptococcus pneumoniae
-
ADP + phosphate + ferrichrome/in
-
?
ATP + H2O + ferrioxamine B/out Streptococcus pneumoniae
-
ADP + phosphate + ferrioxamine B/in
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
R6 strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FhuD protein, gel filtration Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
ABC transporter system characterized, albomycin and salmycin sensitivity assays described, ferrichrome and ferrioxamine B transport assays applied, active transport of albomycin by the ABC transporter shown to mediate the high antibiotic efficacy of Streptococcus pneumoniae Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + albomycin/out
-
Streptococcus pneumoniae ADP + phosphate + albomycin/in
-
?
ATP + H2O + ferrichrome/out
-
Streptococcus pneumoniae ADP + phosphate + ferrichrome/in
-
?
ATP + H2O + ferrioxamine B/out
-
Streptococcus pneumoniae ADP + phosphate + ferrioxamine B/in
-
?
ATP + H2O + salmycin/out
-
Streptococcus pneumoniae ADP + phosphate + salmycin/in
-
?