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Literature summary for 7.2.2.14 extracted from

  • Subramani, S.; Perdreau-Dahl, H.; Morth, J.P.
    The magnesium transporter A is activated by cardiolipin and is highly sensitive to free magnesium in vitro (2016), eLife, 5, e11407 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.14

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin
-
 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D373N inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP-AlF4- less than 10% residual activity at 1 mM Escherichia coli
AlF4- less than 10% residual activity at 1 mM Escherichia coli
Ca2+
-
 Escherichia coli
Co2+
-
 Escherichia coli
Mg2+ the enzyme is highly sensitive to free magnesium in vitro and is strongly inhibited above 1 mM Escherichia coli
MgF42- about 70% residual activity at 1 mM Escherichia coli
Mn2+
-
 Escherichia coli
additional information not inhibited by azide Escherichia coli
Ni2+ non-competitive inhibitor Escherichia coli
vanadate about 25% residual activity at 1 mM Escherichia coli
Zn2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 Mg2+[side 1] with 3 or 6 mM ATP, at pH 7.0 and 37°C Escherichia coli
0.015
-
 Mg2+[side 1] with 1 mM ATP, at pH 7.0 and 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme is activated when the free Mg2+ concentration is reduced below 0.01 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Mg2+[side 1] Escherichia coli
-
 ADP + phosphate + Mg2+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABB8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Mg2+[side 1]
-
 Escherichia coli ADP + phosphate + Mg2+[side 2]
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
magnesium transporter A
-
 Escherichia coli
MgtA
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
 Mg2+[side 1] with 3 or 6 mM ATP, at pH 7.0 and 37°C Escherichia coli
23
-
 Mg2+[side 1] with 1 mM ATP, at pH 7.0 and 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.005
-
 at pH 7.0 and 37°C Escherichia coli Zn2+
0.037
-
 at pH 7.0 and 37°C Escherichia coli Co2+
0.1
-
 at pH 7.0 and 37°C Escherichia coli Ni2+
0.5
-
 at pH 7.0 and 37°C Escherichia coli Ca2+
1
-
 at pH 7.0 and 37°C Escherichia coli Mn2+

General Information

General Information Comment Organism
metabolism the enzyme acts as a sensor as well as a transporter of Mg2+ Escherichia coli