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Literature summary for 7.2.2.14 extracted from

  • Hattori, M.; Tanaka, Y.; Fukai, S.; Ishitani, R.; Nureki, O.
    Crystal structure of the MgtE Mg2+ transporter (2007), Nature, 448, 1072-1075.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mgtE, overexpression of N-terminally His-tagged full-length enzyme and cytosolic domain in Escherichia coli strain C41 (DE3) Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
selenomethionine-labeled full-length enzyme and soluble cytosolic domain in presence and absence of Mg2+, 10 mg/ml full-length protein with 0.1% dodecylmaltoside, by vapour diffusion over 32-34% 2-methyl-2,4-pentanediol, 40 mM magnesium acetate, and 100 mM MES, pH 6.0, cytosolic domain with bound Mg2+ by vapour diffusion over solutions containing 18-22% PEG 400, 0.2M MgCl2, and 0.1M HEPES, pH 7.4, or cytosolic domain in the absence of Mg2+ in 20% PEG 3350, 0.2 M ammonium acetate, and 0.5% octyl-beta-D-glucopyranoside, X-ray diffraction structure determination at 2.3-3.9 A resolution, multiple anomaleous dispersion method Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the transmembrane helices are connected by 5 loops, structure, overview Thermus thermophilus 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Mg2+/out Thermus thermophilus Mg21 homeostasis mechanism model, overview ADP + phosphate + Mg2+/in
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?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SMG8 gene mgtE
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Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged cytosolic domain by nickel affinity chromatography, tag cleavage by thrombin, anion exchange chromatography and gel filtration, recombinant N-terminally His-tagged full-length MgtE from Escherichia coli strain C41 (DE3) by nickel affinity chromatography and gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Mg2+/out Mg21 homeostasis mechanism model, overview Thermus thermophilus ADP + phosphate + Mg2+/in
-
?
ATP + H2O + Mg2+/out transport of the fully hydrated Mg2+ ion, one Mg2+ is bound to conserved Asp432 within the solvent-accessible pore of the transmembrane segement, four Mg2+ ions are bound at the interface between the connecting helices and the other domains, Mg2+ binding structure, overview Thermus thermophilus ADP + phosphate + Mg2+/in
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?

Synonyms

Synonyms Comment Organism
Mg2+ transporter
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Thermus thermophilus
MgtE
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Thermus thermophilus