Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzyme, the arginine rich motif of Mrs2p is not essential for the splicing of group II introns | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D235R | loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview | Saccharomyces cerevisiae |
D244K | loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview | Saccharomyces cerevisiae |
M1301 | gain-of-function mutation, random mutagenesis | Saccharomyces cerevisiae |
additional information | gain-of-function mutations, obtained upon random mutagenesis, map to the conserved primary sequences blocking in the central part of Mrs2, while site-directed mutations in several conserved sequences reduce Mrs2p-mediated Mg2+ uptake and decrease group II intron splicing both resulting in reduced steady-state Mg2+ concentrations, overview | Saccharomyces cerevisiae |
R173E | loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | Mrs2p contains two adjacent transmembrane domains near the C terminus of Mrs2p one of which ends with a F/Y-G-M-N motif | Saccharomyces cerevisiae | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | - |
ADP + phosphate + Mg2+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q02783 | nuclear gene MRS2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | - |
Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | two coiled-coil domains and several conserved primary sequences block in the central part of Mrs2p | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
mitochondrial Mg2+ channel protein | - |
Saccharomyces cerevisiae |
More | the enzyme belongs to the Mrs2 protein family | Saccharomyces cerevisiae |
Mrs2p | - |
Saccharomyces cerevisiae |