Literature summary for 7.2.2.14 extracted from

Payandeh, J.; Pai, E.F.
A structural basis for Mg2+ homeostasis and the CorA translocation cycle (2006), EMBO J., 25, 3762-3773.

Crystallization (Commentary)

Crystallization (Comment)	Organism
free wild-type CorA, CorA1-351, and SeMet-AfCorA1-263, in complex with several different metal ions, 15 mg/ml purified CorA1-351 in 20 mM Tris pH 8.0, 100 mM NaCl, 1 mM TCEP, with or without 0.026% n-dodecyl-beta-D-maltoside, with precipitant vapor-diffusion over 20% PEG 400, 0.2 M CaCl2, 0.1 M HEPES, pH 7.0, 40 mg/ml native and SeMet-AfCorA1-263 from 1.3 M ammonium sulfate, 10 mM CoCl2, 0.1 M HEPES, pH 7.4, X-ray diffraction structure determination and analysis at 3.7 A resolution	Thermotoga maritima

Crystallization (Comment)

Organism

free wild-type CorA, CorA1-351, and SeMet-AfCorA1-263, in complex with several different metal ions, 15 mg/ml purified CorA1-351 in 20 mM Tris pH 8.0, 100 mM NaCl, 1 mM TCEP, with or without 0.026% n-dodecyl-beta-D-maltoside, with precipitant vapor-diffusion over 20% PEG 400, 0.2 M CaCl2, 0.1 M HEPES, pH 7.0, 40 mg/ml native and SeMet-AfCorA1-263 from 1.3 M ammonium sulfate, 10 mM CoCl2, 0.1 M HEPES, pH 7.4, X-ray diffraction structure determination and analysis at 3.7 A resolution

Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Mg2+/out	Thermotoga maritima	-	ADP + phosphate + Mg2+/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WZ31	gene corA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Mg2+/out	-	Thermotoga maritima	ADP + phosphate + Mg2+/in	-	?
ATP + H2O + Mg2+/out	One metal binds near the universally conserved GMN motif, apparently stabilized within the transmembrane region discriminating between the size and preferred coordination geometry of hydrated substrates. CorA achieves specificity by requiring the sequential dehydration of substrates. Ten metal sites are located within the cytoplasmic funnel domain and linked to long extensions of the pore helices regulating the transport status of CorA, this region is an intrinsic divalent cation sensor functioning as a Mg2+-specific homeostatic molecular switch, overview	Thermotoga maritima	ADP + phosphate + Mg2+/in	-	?

Subunits

Subunits	Comment	Organism
More	structural basis for Mg2+ homeostasis and the CorA translocation cycle, open state model and two-state model, overview	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
CorA	-	Thermotoga maritima
CorA Mg2þ transporter homologue	-	Thermotoga maritima