Literature summary for 7.2.2.13 extracted from

Ogawa, H.; Shinoda, T.; Cornelius, F.; Toyoshima, C.
Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain (2009), Proc. Natl. Acad. Sci. USA, 106, 13742-13747.

Crystallization (Commentary)

Crystallization (Comment)	Organism
Na+,K+-ATPase with bound inhibitor ouabain fixed in a state analogous to E2-2K+Pi with a stable phosphate analog MgF4 2-, usage of the crystallization buffer containing 25% glycerol w/v, 5% v/v MPD, 100 mM potassium acetate, 10 mM KCl, 4 mM MgCl2, 4 mM KF, 0.1 mM EGTA, 10 mM glutathione, 0.002 mg/mL 2,6-di-t-butyl-p-cresol, 20 mM MES-Tris, pH 7.0, and 40% w/v PEG 3000, soaking of the crystals in a buffer containing 20 mM ouabain for 5 h, X-ray diffraction structure determination and analysis at 2.8 A resolution	Squalus acanthias

Crystallization (Comment)

Organism

Na+,K+-ATPase with bound inhibitor ouabain fixed in a state analogous to E2-2K+Pi with a stable phosphate analog MgF4 2-, usage of the crystallization buffer containing 25% glycerol w/v, 5% v/v MPD, 100 mM potassium acetate, 10 mM KCl, 4 mM MgCl2, 4 mM KF, 0.1 mM EGTA, 10 mM glutathione, 0.002 mg/mL 2,6-di-t-butyl-p-cresol, 20 mM MES-Tris, pH 7.0, and 40% w/v PEG 3000, soaking of the crystals in a buffer containing 20 mM ouabain for 5 h, X-ray diffraction structure determination and analysis at 2.8 A resolution

Squalus acanthias

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	cardiac glycosides are efficient inhibitors of the Na+,K+-ATPase	Squalus acanthias
Ouabain	a cardiac glycoside derived from Strophanthus gratus, ouabain is deeply inserted into the transmembrane domain with the lactone ring very close to the bound K+. Low-affinity ouabain-bound state due to antagonism between ouabain and K+. The closure of the binding cavity confers a high affinity, homology model for the high affinity state, structures, overview. Binding of sugar moiety and steroid core, overveiw	Squalus acanthias

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Squalus acanthias	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Squalus acanthias

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Na+/in + K+/out	Squalus acanthias	-	ADP + phosphate + Na+/out + K+/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Squalus acanthias	Q4H132	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
rectal gland	-	Squalus acanthias	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Na+/in + K+/out	-	Squalus acanthias	ADP + phosphate + Na+/out + K+/in	-	?

Synonyms

Synonyms	Comment	Organism
Na+,K+-ATPase	-	Squalus acanthias
sodium-potassium pump	-	Squalus acanthias

General Information

General Information	Comment	Organism
physiological function	Na+,K+-ATPase is responsible for establishing Na+ and K+ concentration gradients across the plasma membrane and therefore plays an essential role in, for instance, generating action potentials	Squalus acanthias