Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ouabain | pretreatment of HK-2 cells with ouabain increases the Na+K+ATPase activity and inhibits the inhibitory effect of the D1 receptor on enzyme levels and activity, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fenoldopam | induces inhibition of Na+ ?K+-ATPase activity in HK-2 cells. Ouabain protects against the cyclic adenosine monophosphate accumulation and Na+ ?K+-ATPase inhibition induced by the D1 receptor agonist fenoldopam in HK-2 cells. Chronic ouabain treatment decreases the protein and mRNA expression levels of the D1 receptor and increases the basal phosphorylation of the D1 receptor in HK-2 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in + K+/out | Homo sapiens | - |
ADP + phosphate + Na+/out + K+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HK-2 cell | proximal tubule cell line | Homo sapiens | - |
kidney | - |
Homo sapiens | - |
proximal tubule | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.133 | - |
enzyme activity in HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
0.183 | - |
enzyme activity in HK-2 cells, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
0.25 | - |
enzyme activity in ouabain-pretreated HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
0.267 | - |
enzyme activity in ouabain-pretreated HK-2 cells, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in + K+/out | - |
Homo sapiens | ADP + phosphate + Na+/out + K+/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+/K+-ATPase | - |
Homo sapiens |
renal sodium-potassium pump | - |
Homo sapiens |