Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | odoroside A and ouabain potently reduce NF-kappaB-inducible de novo protein synthesis, largely due to its ability to block Na+-dependent transport of amino acids across the plasma membrane, but not to interfering with the translation machinery | Homo sapiens | |
odoroside A | i.e. 3beta-O-(beta-D-diginosyl)-14-hydroxy-5beta,14beta-card-20(22)-enolide, isolated from the stems and twigs of Nerum oleander, inhibits the enzyme's ATPase activity | Homo sapiens | |
Ouabain | ouabain does not affect TNF-alpha-induced mRNA expression but inhibits TNF-alpha-induced protein expression | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in + K+/out | Homo sapiens | - |
ADP + phosphate + Na+/out + K+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
lung carcinoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in + K+/out | - |
Homo sapiens | ADP + phosphate + Na+/out + K+/in | - |
? | |
ATP + H2O + Na+/in + Rb+/out | measurement of enzyme activity by ouabain-sensitive 86Rb+ uptake | Homo sapiens | ADP + phosphate + Na+/out + Rb+/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+/K+-ATPase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0012 | - |
pH 7.0, 37°C | Homo sapiens | odoroside A | |
0.0016 | - |
pH 7.0, 37°C | Homo sapiens | Ouabain |
General Information | Comment | Organism |
---|---|---|
additional information | odoroside A and ouabain potently reduce NF-kappaB-inducible de novo protein synthesis, largely due to its ability to block Na+-dependent transport of amino acids across the plasma membrane, but not to interfering with the translation machinery | Homo sapiens |