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Literature summary for 7.2.2.13 extracted from

  • Benziane, B.; Bjoernholm, M.; Lantier, L.; Viollet, B.; Zierath, J.R.; Chibalin, A.V.
    AMP-activated protein kinase activator A-769662 is an inhibitor of the Na+-K+-ATPase (2009), Am. J. Physiol. Cell Physiol., 297, C1554-C1566.
    View publication on PubMed
Search for more literature for 7.2.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in HEK-293 cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
A-769662 inhibits the Na+-K+-ATPase transport activity and cell surface abundance in L6 cells, which is independent of AMP kinase activation, overview Rattus norvegicus
Ouabain
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-
plasma membrane
-
 Rattus norvegicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Rattus norvegicus
Ca2+ activates Rattus norvegicus
Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Rattus norvegicus
-
 ADP + phosphate + Na+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of the alpha-subunit triggers the Na+-K+-ATPase activity in endocytosis and exocytosis, A-769662 inhibits the Na+-K+-ATPase cell surface abundance in L6 cells by decreasing the phosphorylation of the alpha-subunit independently of the AMP kinase activity Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from kidney cortex, recombinant His-tagged enzyme from HEK-293 cells by nickel affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
L-6 myoblast cell muscle cell line, A-769662 inhibits the Na+-K+-ATPase cell surface abundance in L6 cells by decreasing the phosphorylation of the alpha-subunit Rattus norvegicus
-
masseter
-
 Rattus norvegicus
-
medial pterygoid muscle
-
 Rattus norvegicus
-
myotube
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
 Rattus norvegicus ADP + phosphate + Na+/out + K+/in
-
 ?
ATP + H2O + Na+/in + Rb+/out measurement of enzyme activity by ouabain-sensitive Rb+ uptake Rattus norvegicus ADP + phosphate + Na+/out + Rb+/in
-
 ?

Synonyms

Synonyms Comment Organism
Na+-K+-ATPase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus