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Literature summary for 7.2.2.13 extracted from

  • Horisberger, J.D.; Kharoubi-Hess, S.; Guennoun, S.; Michielin, O.
    The Fourth Transmembrane Segment of the Na,K-ATPase a Subunit: A systematic mutagenesis study (2004), J. Biol. Chem., 279, 29542-29550.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Xenopus laevis oocyte Rhinella marina
in pSD5 vector, expression of proteins in Xenopus laevis oocytes Rhinella marina

Protein Variants

Protein Variants Comment Organism
A330C difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range, negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range Rhinella marina
A330C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
A338C difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range Rhinella marina
A338C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
C111S little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
C111S used as control Rhinella marina
E334C negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range, K+-activated current with an amplitude half of the oubain-sensitive current in the high negative membrane potential range Rhinella marina
E334C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
F323C small ouabain-sensitive current over the whole membrane potential range, significant K+-induced inward curve mostly at negative membrane potential range Rhinella marina
F323C K+ induced current, I_K in nA, lower than control(negativ) - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
G326C inhibition by 2-aminoethyl-methanethiosulfonate Rhinella marina
G326C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
G335C identical and strongly voltage-dependent I/V relationship of the K+-induced and ouabain sensitive currents Rhinella marina
G335C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
I322C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
I322C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
I325C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
I325C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
I327C difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range Rhinella marina
I327C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
I328C negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range Rhinella marina
I328C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
L324C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
L324C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control Rhinella marina
L336C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
L336C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
L337C K+ induced current, I_K in nA, lower (negative) than control - ouabain-sensitive current, I_ouab in nA, lower (negative) than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
L344C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
L344C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
N331C difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range Rhinella marina
N331C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control Rhinella marina
P333C inhibition by 2-aminoethyl-methanethiosulfonate Rhinella marina
P333C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
T339C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower control Rhinella marina
T341C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control Rhinella marina
V321C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
V321C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control Rhinella marina
V329C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
V329C K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control Rhinella marina
V332C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
V332C K+ induced current, I_K in nA, similar to control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control Rhinella marina
V340C little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range Rhinella marina
V340C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina
V342C K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control Rhinella marina

Inhibitors

Inhibitors Comment Organism Structure
2-aminoethyl-methanethiosulfonate inhibition of mutants F323C, P333C, E334C, G335C, I322C, G326C and A330C Rhinella marina
Ouabain g-strophanthin Rhinella marina

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Rhinella marina 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + Na+/out Rhinella marina
-
ATP + H2O + Na+/in
-
?
ATP + H2O + K+/in Rhinella marina
-
ADP + phosphate + K+/out
-
?

Organism

Organism UniProt Comment Textmining
Rhinella marina
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + Na+/out
-
Rhinella marina ATP + H2O + Na+/in
-
?
ATP + H2O + K+/in
-
Rhinella marina ADP + phosphate + K+/out
-
?

Subunits

Subunits Comment Organism
dimer alpha subunit, main subunit - beta subunit, glycoprotein Rhinella marina

Synonyms

Synonyms Comment Organism
Na,K-ATPase
-
Rhinella marina