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Literature summary for 7.2.2.12 extracted from

  • Schurig-Briccio, L.A.; Gennis, R.B.
    Characterization of the PIB-type ATPases present in Thermus thermophilus (2012), J. Bacteriol., 194, 4107-4113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 (DE3) cells Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus
Cu2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus
Zn2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Zn2+/in
-
 Thermus thermophilus ADP + phosphate + Zn2+/out
-
 ?
ATP + H2O + Zn2+/in
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ADP + phosphate + Zn2+/out
-
 ?

Synonyms

Synonyms Comment Organism
zinc-cadmium transporter
-
 Thermus thermophilus
Zn2+/Cd2+-ATPase
-
 Thermus thermophilus