Literature summary for 7.2.2.12 extracted from

Leitenmaier, B.; Witt, A.; Witzke, A.; Stemke, A.; Meyer-Klaucke, W.; Kroneck, P.M.; Kuepper, H.
Biochemical and biophysical characterisation yields insights into the mechanism of a Cd/Zn transporting ATPase purified from the hyperaccumulator plant Thlaspi caerulescens (2011), Biochim. Biophys. Acta, 1808, 2591-2599.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	activates at 0.00003 mM, the level of activation does not change much until a concentration of 0.001 mM	Noccaea caerulescens
Cu2+	activates at 0.00003 mM	Noccaea caerulescens
Zn2+	activates at 0.00003 mM, the level of activation does not change much until a concentration of 0.0003 mM	Noccaea caerulescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 60000, SDS-PAGE	Noccaea caerulescens

Organism

Organism	UniProt	Comment	Textmining
Noccaea caerulescens	Q70LF4	ecotype Ganges	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-IDA metal affinity column chromatography, gel filtration	Noccaea caerulescens

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Noccaea caerulescens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Zn2+/in	-	Noccaea caerulescens	ADP + phosphate + Zn2+/out	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Noccaea caerulescens

Synonyms

Synonyms	Comment	Organism
Cd/Zn transporting ATPase	-	Noccaea caerulescens
HMA4	-	Noccaea caerulescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	-	Noccaea caerulescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
300	-	ATP	in the presence of Zn2+ or Cd2+, in 0.6 M Tris, 2 M NaCl and 0.1 M MgCl2, pH 8.0, at 30°C	Noccaea caerulescens

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Release 2023.1 (January 2023)