Cloned (Comment) | Organism |
---|---|
expression of wild-type HMA2 and mutant derivatives in transgenic hma2,hma4, Zn-deficient plants from the HMA2 promoter, quantitative realtime PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of mutant derivatives, with and without a C-terminal GFP tag, from the HMA2 promoter in transgenic hma2,hma4, Zn-deficient plants. The deletion mutant lacking the C-terminal 244 amino acids rescues most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development. Root-to-shoot Cd translocation is fully rescued. The GFP-tagged derivative is partially mislocalized in the root pericycle cells in which it is expressed. Deletion derivatives lacking the C-terminal 121 and 21 amino acids rescue all phenotypes and localize normally. N-terminal domain mutants localize normally but fail to complement the hma2,hma4 phenotypes, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Zn2+/in | Arabidopsis thaliana | the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein | ADP + phosphate + Zn2+/out | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col-0 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pericycle | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Zn2+/in | the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein | Arabidopsis thaliana | ADP + phosphate + Zn2+/out | - |
? | |
ATP + H2O + Zn2+/in | HMA2 has N- and C-terminal domains that can bind Zn ions with high affinity | Arabidopsis thaliana | ADP + phosphate + Zn2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HMA2 | - |
Arabidopsis thaliana |
More | the enzyme is a member of the P1B subfamily of the ATPases | Arabidopsis thaliana |
Zn/Cd-transporting ATPase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |