Cloned (Comment) | Organism |
---|---|
into the pTRCHisA vector for expression in the Escherichia coli TOP 10 strain | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D714M | significant metal-independent ATPase activity, poor phosphorylation by ATP and Pi | Escherichia coli |
K693N | very low activity, no Cu2+-dependent phosphorylation with ATP, hyperphosphorylation by Pi, altered metal sensitivity of phosphorylation by Pi | Escherichia coli |
K693N/D714M | very low activity and phosphorylation with ATP, almost normal phosphorylation with Pi, altered metal senstivity of Pi phosphorylation | Escherichia coli |
SAAS | mutation of two the two cysteines to serines in the CAAC motif near the N-terminus, ATPase activity 50%, diminished phosphorylation, faster dephosphorylation | Escherichia coli |
SPS | mutation of two the two cysteines to serines in the CPC motif in transmembrane helix 6, inactive, very low phosphorylation | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
on PAA gels the protein is present in three forms, as a monomer with an apparent mass of 92 kDa, as a dimer of 190 kDa and as a proteolytic fragment of 67 kDa | Escherichia coli |
80000 | - |
monomer, determined by SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | Escherichia coli | - |
ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Pb2+/in | Escherichia coli | - |
ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | Escherichia coli | - |
ADP + phosphate + Zn2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the membrane fractions of the cells are isolated | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 20% glycerol, 2 mM beta-mercaptoethanol, 0.5 mM PMSF | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | - |
Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Pb2+/in | - |
Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | - |
Escherichia coli | ADP + phosphate + Zn2+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | on PAA gels the protein is present in three forms, as a monomer with an apparent mass of 92 kDa, as a dimer of 190 kDa and as a proteolytic fragment of 67 kDa | Escherichia coli |
monomer | on PAA gels the protein is present in three forms, as a monomer with an apparent mass of 92 kDa, as a dimer of 190 kDa and as a proteolytic fragment of 67 kDa | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
zinc-transporting P-type ATPase | - |
Escherichia coli |
Zn2+ -exporting ATPase | - |
Escherichia coli |
ZntA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
phosphorylation assays are carried out on ice and room temperature | Escherichia coli |
37 | - |
ATPase activity assay | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
ATPase activity assay | Escherichia coli |