Protein Variants | Comment | Organism |
---|---|---|
D436N | the mutant is completely inactive with respect to both in vivo resistance and ATP hydrolysis activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
ATP | pH 7.0, 37°C | Escherichia coli | |
0.0042 | - |
Cd2+ | pH 7.0, 37°C | Escherichia coli | |
0.0054 | - |
Zn2+ | pH 7.0, 37°C | Escherichia coli | |
0.0064 | - |
Pb2+ | pH 7.0, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
monomeric form, SDS-PAGE | Escherichia coli |
160000 | - |
dimeric form, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
The activity with Ni2+, Co2+ and Cu2+ are extremely low, 10-20fold lower compared with the values obtained with Pb2+, Zn2+ and Cd2+. | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | dephosphorylation with EDTA | Escherichia coli | ADP + phosphate + Cd2+/out | - |
r | |
ATP + H2O + Co2+/in | - |
Escherichia coli | ADP + phosphate + Co2+/out | - |
? | |
ATP + H2O + Cu2+/in | - |
Escherichia coli | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Ni2+/in | - |
Escherichia coli | ADP + phosphate + Ni2+/out | - |
? | |
ATP + H2O + Pb2+/in | dephosphorylation with EDTA | Escherichia coli | ADP + phosphate + Pb2+/out | - |
r | |
ATP + H2O + Zn2+/in | dephosphorylation with EDTA | Escherichia coli | ADP + phosphate + Zn2+/out | - |
r | |
additional information | not: Cu+ and Ag+ | Escherichia coli | ? | - |
? |