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Literature summary for 7.2.2.10 extracted from

  • Clausen, J.D.; Andersen, J.P.
    Glutamate 90 at the luminal ion gate of sarcoplasmic reticulum Ca2+-ATPase is critical for Ca2+ binding on both sides of the membrane (2010), J. Biol. Chem., 285, 20780-20792.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.10

Protein Variants

Protein Variants Comment Organism
E90A the mutant shows a reduction of the apparent affinity for luminal Ca2+ and exhibits 19% of wild type activity Oryctolagus cuniculus
E90L the mutant shows a reduction of the apparent affinity for luminal Ca2+ and exhibits less than 10% of wild type activity Oryctolagus cuniculus
E90R the mutation allows E2P formation from phosphate even at luminal Ca2+ concentrations much too small to support phosphorylation in wild type. The mutant with less than 10% of wild type activity further displays a blocked dephosphorylation of E2P and an increased rate of conversion of the ADP-sensitive E1P phosphoenzyme intermediate to ADP-insensitive E2P as well as insensitivity of the E2-BeF3-complex to luminal Ca2+ Oryctolagus cuniculus
K297A the mutant exhibits 87% of wild type activity Oryctolagus cuniculus
S72A the mutant exhibits 80% of wild type activity Oryctolagus cuniculus
S72R the mutation allows E2P formation from phosphate even at luminal Ca2+ concentrations much too small to support phosphorylation in wild type. The mutant with less than 10% of wild type activity further displays a blocked dephosphorylation of E2P and an increased rate of conversion of the ADP-sensitive E1P phosphoenzyme intermediate to ADP-insensitive E2P as well as insensitivity of the E2-BeF3-complex to luminal Ca2+ Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus B6CAM1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/out
-
 Oryctolagus cuniculus ADP + phosphate + Ca2+/in
-
 ?

Synonyms

Synonyms Comment Organism
sarcoplasmic reticulum Ca2+-ATPase
-
 Oryctolagus cuniculus
SERCA1a isoform Oryctolagus cuniculus