Crystallization (Comment) | Organism |
---|---|
0.005 ml of 2 mg/ml purified K-ring in 20 mM Tris-HCl, pH 7.5, 100 mM LiCl, 10% glycerol, and 0.32 mM n-dodecyl-beta-D-maltoside by sitting drop vapor diffusion method, mixing with 0.005 ml of reservoir solution consisting of 100 mM Tris-HCl, pH 7.5, 4% glycerol, 240 mM potassium citrate, 1.2 mM undecylmaltoside, and 34% PEG 400, at 20°C, in presence or absence of 10 mM NaCl, first appearance after 3 days, maximal size after 3 weeks, X-ray diffraction structure determination and analysis at 2.8 A resolution | Enterococcus hirae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H+ | competitively inhibits binding of Na+ to the K-ring of the enzyme, binding structure, overview | Enterococcus hirae | |
Li+ | competitively inhibits binding of Na+ to the K-ring of the enzyme, binding structure, overview | Enterococcus hirae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Na+ binding kinetics | Enterococcus hirae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in | Enterococcus hirae | - |
ADP + phosphate + Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus hirae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the V-ATPase and isolation of the K-ring by by anion exchange chromatography and gel filtration | Enterococcus hirae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in | - |
Enterococcus hirae | ADP + phosphate + Na+/out | - |
? | |
ATP + H2O + Na+/in | the purified rotor ring, i.e. K-ring, of the V-ATPase binds one Na+ ion per K-monomer with high affinity. Interaction with the stator subunit, i.e. I-subunit, is essential for Na binding to or release from the K-ring, respectively, ion translocation model, overview | Enterococcus hirae | ADP + phosphate + Na+/out | - |
? | |
additional information | K+, Tl+, Rb+, and Cs+ cannot substitute Na+ | Enterococcus hirae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+-transporting V-ATPase | - |
Enterococcus hirae |
vacuole-type ATPase | - |
Enterococcus hirae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Enterococcus hirae |