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Literature summary for 7.2.2.1 extracted from
- The ntp operon encoding the Na+ V-ATPase of the thermophile Caloramator fervidus (2006), Arch. Microbiol., 186, 513-517.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ntp operon, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Caloramator fervidus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Caloramator fervidus | 5829 | - |
| membrane | - |
Caloramator fervidus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
x * 10000, G subunit, SDS-PAGE, x * 11600, about, G subunit, sequence calculation | Caloramator fervidus |
| 11600 | - |
x * 10000, G subunit, SDS-PAGE, x * 11600, about, G subunit, sequence calculation | Caloramator fervidus |
| 12000 | - |
x * 12000, F subunit, SDS-PAGE, x * 12300, about, F subunit, sequence calculation | Caloramator fervidus |
| 12300 | - |
x * 12000, F subunit, SDS-PAGE, x * 12300, about, F subunit, sequence calculation | Caloramator fervidus |
| 17000 | - |
x * 17000, K subunit, SDS-PAGE, x * 17600, about, K subunit, sequence calculation | Caloramator fervidus |
| 17600 | - |
x * 17000, K subunit, SDS-PAGE, x * 17600, about, K subunit, sequence calculation | Caloramator fervidus |
| 22700 | - |
x * 26000, E subunit, SDS-PAGE, x * 22700, about, E subunit, sequence calculation | Caloramator fervidus |
| 24300 | - |
x * 25500, D subunit, SDS-PAGE, x * 24300, about, D subunit, sequence calculation | Caloramator fervidus |
| 25500 | - |
x * 25500, D subunit, SDS-PAGE, x * 24300, about, D subunit, sequence calculation | Caloramator fervidus |
| 26000 | - |
x * 26000, E subunit, SDS-PAGE, x * 22700, about, E subunit, sequence calculation | Caloramator fervidus |
| 37000 | - |
x * 37000, C subunit, SDS-PAGE, x * 38400, about, C subunit, sequence calculation | Caloramator fervidus |
| 38400 | - |
x * 37000, C subunit, SDS-PAGE, x * 38400, about, C subunit, sequence calculation | Caloramator fervidus |
| 51000 | - |
x * 51000, B subunit, SDS-PAGE, x * 51200, about, B subunit, sequence calculation | Caloramator fervidus |
| 51200 | - |
x * 51000, B subunit, SDS-PAGE, x * 51200, about, B subunit, sequence calculation | Caloramator fervidus |
| 66000 | - |
x * 66000, A subunit, SDS-PAGE, x * 66000, about, A subunit, sequence calculation | Caloramator fervidus |
| 72300 | - |
x * 72300, I subunit, SDS-PAGE, x * 72300, about, I subunit, sequence calculation | Caloramator fervidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Caloramator fervidus | the V-type ATPase pumps Na+ ions out of the cell against the sodium ion motive force at the expense of the hydrolysis of ATP. The enzyme complex consists of an integral membrane part V0 that is responsible for the translocation of the Na+ ions and a cytoplasmic, membrane associated part V1 that hydrolyzes ATP, it functions as two coupled molecular motors | ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caloramator fervidus | Q2EQR4 | gene ntpD; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpD | - |
| Caloramator fervidus | Q2EQR5 | gene ntpB; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpB | - |
| Caloramator fervidus | Q2EQR6 | gene ntpA; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpA | - |
| Caloramator fervidus | Q2EQR7 | gene ntpG; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpG | - |
| Caloramator fervidus | Q2EQR8 | gene ntpC; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpC | - |
| Caloramator fervidus | Q2EQR9 | gene ntpE; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpE | - |
| Caloramator fervidus | Q2EQS0 | gene ntpK; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpK | - |
| Caloramator fervidus | Q2EQS1 | gene ntpI; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpI | - |
| Caloramator fervidus | Q2EQS2 | gene ntpF; strain ATCC 43024, ntpFIKECGABD operon contains the structural genes coding for the nine subunits of the enzyme complex, gene ntpF | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Caloramator fervidus | ADP + phosphate | - |
ir | |
| ATP + H2O | the V-type ATPase pumps Na+ ions out of the cell against the sodium ion motive force at the expense of the hydrolysis of ATP. The enzyme complex consists of an integral membrane part V0 that is responsible for the translocation of the Na+ ions and a cytoplasmic, membrane associated part V1 that hydrolyzes ATP, it functions as two coupled molecular motors | Caloramator fervidus | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 10000, G subunit, SDS-PAGE, x * 11600, about, G subunit, sequence calculation | Caloramator fervidus |
| ? | x * 12000, F subunit, SDS-PAGE, x * 12300, about, F subunit, sequence calculation | Caloramator fervidus |
| ? | x * 17000, K subunit, SDS-PAGE, x * 17600, about, K subunit, sequence calculation | Caloramator fervidus |
| ? | x * 25500, D subunit, SDS-PAGE, x * 24300, about, D subunit, sequence calculation | Caloramator fervidus |
| ? | x * 26000, E subunit, SDS-PAGE, x * 22700, about, E subunit, sequence calculation | Caloramator fervidus |
| ? | x * 37000, C subunit, SDS-PAGE, x * 38400, about, C subunit, sequence calculation | Caloramator fervidus |
| ? | x * 51000, B subunit, SDS-PAGE, x * 51200, about, B subunit, sequence calculation | Caloramator fervidus |
| ? | x * 66000, A subunit, SDS-PAGE, x * 66000, about, A subunit, sequence calculation | Caloramator fervidus |
| ? | x * 72300, I subunit, SDS-PAGE, x * 72300, about, I subunit, sequence calculation | Caloramator fervidus |
| More | the enzyme complex consists of an integral membrane part V0 and a cytoplasmic, membrane-associated part V1, V0 is built of subunits K and I, V1 consists of seven subunits, termed A, B, C, D, E, F, and G, to determine the identity of the proteins in two pairs of subunits, the nine subunits of the enzyme complex are separated by tryptic digestion of the protein bands followed by mass spectrometric analysis of the peptides | Caloramator fervidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Na+ V-ATPase | - |
Caloramator fervidus |
| Ntp | - |
Caloramator fervidus |
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