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Literature summary for 7.2.2.1 extracted from

  • Ferguson Scott, F.S.; Keis Stefani, K.S.; Cook Gregory, C.G.
    Biochemical and molecular characterization of a Na+-translocating F1Fo-ATPase from the thermoalkaliphilic bacterium Clostridium paradoxum (2006), J. Bacteriol., 188, 5045-5054.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DH10B [Clostridium] paradoxum

Inhibitors

Inhibitors Comment Organism Structure
Carbonyl cyanide m-chlorophenylhydrazone 0.02 mM, pH 9.0, 75% inhibition with no or low levels of NaCl [Clostridium] paradoxum
N,N'-dicyclohexylcarbodiimide 0.05 mM, at pH 7.0, 60% inhibition in the presence of 50 mM NaCl, no inhibitory effect in the presence of 1mM NaCl at pH 9.0 [Clostridium] paradoxum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 ATP in the presence of Na+ at pH 7. 0 and 9.0 [Clostridium] paradoxum
0.55
-
 ATP Mg2+/ATP ratio at 2:1 [Clostridium] paradoxum
1
-
 ATP in the presence of MgCl2 [Clostridium] paradoxum

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ three to fourfold stimulation of activity at pH 7.0, five to sixfold stimulation of activity at pH 9.0 [Clostridium] paradoxum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8255
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
14184
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
19443
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
20763
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
25447
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
31709
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
50453
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
55667
-
 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum
60000
-
 SDS-PAGE [Clostridium] paradoxum

Organism

Organism UniProt Comment Textmining
[Clostridium] paradoxum
-
 strain DSM 7308
-

Purification (Commentary)

Purification (Comment) Organism
precipitation using polyethylene glycol 6000 [Clostridium] paradoxum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 enzyme from membrane vesicles, at 40°C in 50 mM MOPS buffer (pH 7.5.) [Clostridium] paradoxum
1.1
-
 enzyme after 2.2fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) [Clostridium] paradoxum
6
-
 enzyme after 12fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) [Clostridium] paradoxum
15
-
 enzyme after 30fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) [Clostridium] paradoxum

Storage Stability

Storage Stability Organism
-80°C, 10 mM Tris-HCl pH 8.0 containing 2 mM MgCl2 to a protein concentration of 1.5 mg/ml, stable for at least 2 months [Clostridium] paradoxum
4°C, 50 mM MOPS pH 7.5, 4 days, no activity loss of the native enzyme [Clostridium] paradoxum
4°C, 50 mM MOPS pH 7.5, 5 days, 75% activity loss of the purified enzyme [Clostridium] paradoxum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in
-
 [Clostridium] paradoxum ADP + phosphate + Na+/out
-
 ?

Subunits

Subunits Comment Organism
nonamer 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA [Clostridium] paradoxum

Synonyms

Synonyms Comment Organism
F-type ATP synthase
-
 [Clostridium] paradoxum
Na+-translocating F1FO-ATPase
-
 [Clostridium] paradoxum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.5
-
 [Clostridium] paradoxum