Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DH10B | [Clostridium] paradoxum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carbonyl cyanide m-chlorophenylhydrazone | 0.02 mM, pH 9.0, 75% inhibition with no or low levels of NaCl | [Clostridium] paradoxum | |
N,N'-dicyclohexylcarbodiimide | 0.05 mM, at pH 7.0, 60% inhibition in the presence of 50 mM NaCl, no inhibitory effect in the presence of 1mM NaCl at pH 9.0 | [Clostridium] paradoxum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
ATP | in the presence of Na+ at pH 7. 0 and 9.0 | [Clostridium] paradoxum | |
0.55 | - |
ATP | Mg2+/ATP ratio at 2:1 | [Clostridium] paradoxum | |
1 | - |
ATP | in the presence of MgCl2 | [Clostridium] paradoxum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | three to fourfold stimulation of activity at pH 7.0, five to sixfold stimulation of activity at pH 9.0 | [Clostridium] paradoxum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8255 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
14184 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
19443 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
20763 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
25447 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
31709 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
50453 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
55667 | - |
1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
60000 | - |
SDS-PAGE | [Clostridium] paradoxum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] paradoxum | - |
strain DSM 7308 | - |
Purification (Comment) | Organism |
---|---|
precipitation using polyethylene glycol 6000 | [Clostridium] paradoxum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
enzyme from membrane vesicles, at 40°C in 50 mM MOPS buffer (pH 7.5.) | [Clostridium] paradoxum |
1.1 | - |
enzyme after 2.2fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) | [Clostridium] paradoxum |
6 | - |
enzyme after 12fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) | [Clostridium] paradoxum |
15 | - |
enzyme after 30fold purification, at 40°C in 50 mM MOPS buffer (pH 7.5) | [Clostridium] paradoxum |
Storage Stability | Organism |
---|---|
-80°C, 10 mM Tris-HCl pH 8.0 containing 2 mM MgCl2 to a protein concentration of 1.5 mg/ml, stable for at least 2 months | [Clostridium] paradoxum |
4°C, 50 mM MOPS pH 7.5, 4 days, no activity loss of the native enzyme | [Clostridium] paradoxum |
4°C, 50 mM MOPS pH 7.5, 5 days, 75% activity loss of the purified enzyme | [Clostridium] paradoxum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in | - |
[Clostridium] paradoxum | ADP + phosphate + Na+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
nonamer | 1 * 14184 + 1 * 25447 + 1 * 8255 + 1 * 19443 + 1 * 20763 + 1 * 55667 + 1 * 31709 + 1 * 50453, calculated from sequence of cDNA | [Clostridium] paradoxum |
Synonyms | Comment | Organism |
---|---|---|
F-type ATP synthase | - |
[Clostridium] paradoxum |
Na+-translocating F1FO-ATPase | - |
[Clostridium] paradoxum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.5 | - |
[Clostridium] paradoxum |