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Literature summary for 7.2.1.2 extracted from

  • Biegel, E.; Müller, V.
    Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase (2010), Proc. Natl. Acad. Sci. USA, 107, 18138-18142.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.1.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition of Na+ transport at 0.5 mM Acetobacterium woodii
Ag+ complete inhibition of Na+ transport at 0.1 mM Acetobacterium woodii
Cu2+ complete inhibition of Na+ transport at 0.08 mM Acetobacterium woodii
diphenyleniodonium chloride complete inhibition of Na+ transport at 0.04 mM Acetobacterium woodii
diphenyliodonium chloride complete inhibition of Na+ transport at 0.04 mM Acetobacterium woodii
additional information the enzyme complex is inhibited by sodium ionophores but not protonophores, demonstrating a direct coupling of the ferredoxin:NAD+ oxidoreductase activity to Na+ transport Acetobacterium woodii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 Na+/in pH 6.0, 30°C Acetobacterium woodii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Acetobacterium woodii 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii electron transfer from reduced ferredoxin to NAD+ as acceptor drives the generation of a transmembrane ion gradient 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in
-
 Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
 ?
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transfer from reduced ferredoxin to NAD+ as acceptor drives the generation of a transmembrane ion gradient Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
 ?

Synonyms

Synonyms Comment Organism
Na+-translocating ferredoxin:NAD+ oxidoreductase
-
 Acetobacterium woodii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Acetobacterium woodii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Acetobacterium woodii

Cofactor

Cofactor Comment Organism Structure
flavin iron–sulfur and flavin-containing electron transport complex Acetobacterium woodii
iron-sulfur centre iron–sulfur and flavin-containing electron transport complex Acetobacterium woodii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000035
-
 pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity Acetobacterium woodii diphenyleniodonium chloride
0.001
-
 pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity Acetobacterium woodii diphenyliodonium chloride
0.00175
-
 pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity Acetobacterium woodii Cu2+
0.00375
-
 pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity Acetobacterium woodii Ag+
0.155
-
 pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity Acetobacterium woodii 1,10-phenanthroline

General Information

General Information Comment Organism
metabolism the enzyme complex is involved in Na+-motive, anaerobic respiration with caffeate as electron acceptor Acetobacterium woodii