Literature summary for 7.2.1.1 extracted from

Nakayama, Y.; Hayashi, M.; Unemoto, T.
Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus (1998), FEBS Lett., 422, 240-242.

Search for more literature for 7.2.1.1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Vibrio alginolyticus	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19000	-	1 * 50000 + 1 * 30000-35000 + 1 * 32000 + 1 * 20000 + 1 * 19000 + 1 * 46000, SDS-PAGE	Vibrio alginolyticus
20000	-	1 * 50000 + 1 * 30000-35000 + 1 * 32000 + 1 * 20000 + 1 * 19000 + 1 * 46000, SDS-PAGE	Vibrio alginolyticus
21540	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
22470	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
27571	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
32000	-	1 * 50000 + 1 * 30000-35000 + 1 * 32000 + 1 * 20000 + 1 * 19000 + 1 * 46000, SDS-PAGE	Vibrio alginolyticus
45210	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
45274	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
46000	-	1 * 50000 + 1 * 30000-35000 + 1 * 32000 + 1 * 20000 + 1 * 19000 + 1 * 46000, SDS-PAGE	Vibrio alginolyticus
48622	-	1 * 48622 + 1 * 45210 + 1 * 27571 + 1 * 22470 + 1 * 21540 + 1 * 45274, calculated from amino acid sequence	Vibrio alginolyticus
50000	-	1 * 50000 + 1 * 30000-35000 + 1 * 32000 + 1 * 20000 + 1 * 19000 + 1 * 46000, SDS-PAGE	Vibrio alginolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio alginolyticus	Q56586 and Q56587 and Q56582 and Q57095 and Q56589 and Q56584	Q56586: subunit Nqr1 (NqrA), Q56587: subunit Nqr2 (NqrB), Q56582: subunit Nqr3 (NqrC), Q57095: subunit Nqr4 (NqrD), Q56589: subunit Nqr5 (NqrE), and Q56584: subunit Nqr6 (NqrF). The enzyme consists of six subunits encoded by the NQR operon.	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE Sephacel column chromatography, TSK-gel DEAE-5PW column chromatography, and Superdex 200 gel filtration	Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H+ + ubiquinone-1 + n Na+/in	-	Vibrio alginolyticus	NAD+ + ubiquinol-1 + n Na+/out	-	?

Subunits

Subunits	Comment	Organism
heterohexamer	1 * 48622 (NqrA) + 1 * 45210 (NqrB) + 1 * 27571 (NqrC) + 1 * 22470 (NqrD) + 1 * 21540 (NqrE) + 1 * 45274 (NqrF), calculated from amino acid sequence	Vibrio alginolyticus
heterohexamer	1 * 50000 (NqrA) + 1 * 30000-35000 (NqrB) + 1 * 32000 (NqrC) + 1 * 20000 (NqrD) + 1 * 19000 (NqrE) + 1 * 46000 (NqrF), SDS-PAGE	Vibrio alginolyticus

Synonyms

Synonyms	Comment	Organism
Na+-translocating NADH-quinone reductase	-	Vibrio alginolyticus
NQR	-	Vibrio alginolyticus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Vibrio alginolyticus
FMN	-	Vibrio alginolyticus
NADH	-	Vibrio alginolyticus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio alginolyticus	subunit Nqr6 (NqrF), isoelectric focusing	-	4.6
Vibrio alginolyticus	subunit Nqr3 (NqrC), isoelectric focusing	-	4.8
Vibrio alginolyticus	subunit Nqr1(NqrA), isoelectric focusing	-	5.4
Vibrio alginolyticus	subunit Nqr5 (NqrE), isoelectric focusing	-	7
Vibrio alginolyticus	subunit Nqr2 (NqrB), isoelectric focusing	-	8.2
Vibrio alginolyticus	subunit Nqr4 (NqrD), isoelectric focusing	-	9.2

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Release 2023.1 (January 2023)