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Literature summary for 7.2.1.1 extracted from

  • Barquera, B.; Hellwig, P.; Zhou, W.; Morgan, J.E.; Häse, C.C.; Gosink, K.K.; Nilges, M.; Bruesehoff, P.J.; Roth, A.; Lancaster, C.R.D.; Gennis, R.B.
    Purification and characterization of the recombinant Na+-translocating NADH:quinone oxidoreductase from Vibrio cholera (2002), Biochemistry, 41, 3781-3789.
    View publication on PubMed
Search for more literature for 7.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cloned under the regulation of the P(BAD) promoter, successfully expressed in Vibrio cholerae Vibrio cholerae serotype O1
cloned under the regulation of the PBAD promoter and expressed in Vibrio cholera Vibrio cholerae serotype O1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated to Vibrio cholerae serotype O1 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ recombinant enzyme has high specific activity in presence of Na+ Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
 gel filtration Vibrio cholerae serotype O1
360000
-
 it is likely that the protein is a monomer, calculated molecular mass of 215000 Da with bound detergent, gel filtration Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + ubiquinone Vibrio cholerae serotype O1 Na+-translocation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the NADH oxidation is coupled to the pumping of Na+ across the membrane Vibrio cholerae serotype O1 ?
-
 ?
NADH + ubiquinone Na+-translocation Vibrio cholerae serotype O1 NAD+ + ubiquinol
-
 ?
NADH + ubiquinone Na+-translocation Vibrio cholerae serotype O1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Na(+)-translocating NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1
Na+-NQR
-
 Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains one molecule of noncovalently bound FAD Vibrio cholerae serotype O1
FAD contains one molecule of covalently bound FAD, believed to be bound to subunit F Vibrio cholerae serotype O1
FMN enzyme contains 2 molecules of covalently bound FMN Vibrio cholerae serotype O1
FMN the enzyme contains two molecules of covalently bound FMN, covalently bound to subunits NqrB and NqrC Vibrio cholerae serotype O1
NADH
-
 Vibrio cholerae serotype O1
ubiquinone enzyme contains approximately one bound ubiquinone Vibrio cholerae serotype O1