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Literature summary for 7.1.3.1 extracted from

  • Espiau, B.; Lemercier, G.; Ambit, A.; Bringaud, F.; Merlin, G.; Baltz, T.; Bakalara, N.
    A soluble pyrophosphatase, a key enzyme for polyphosphate metabolism in Leishmania (2006), J. Biol. Chem., 281, 1516-1523.
    View publication on PubMed
Search for more literature for 7.1.3.1

Application

Application Comment Organism
drug development VSP1 enzyme an attractive drug target against trypanosomatid parasites Leishmania amazonensis

Cloned(Commentary)

Cloned (Comment) Organism
gene VSP1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli, overexpression of wild-type and mutant enzymes in Leishmania amazonensis Leishmania amazonensis

Protein Variants

Protein Variants Comment Organism
D325E site-directed mutagenesis, inactive mutant, the dominant negative mutant shows the requirement of Leishmania VSP1 functional expression for metacyclogenesis and virulence in mice, overview Leishmania amazonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the structural orientation of Lys78 is very important for the kinetic properties of the enzyme Leishmania amazonensis
0.092
-
 diphosphate 25°C Leishmania amazonensis
0.123
-
 polyphosphate-n28 25°C Leishmania amazonensis
1.153
-
 Triphosphate 25°C Leishmania amazonensis

Localization

Localization Comment Organism GeneOntology No. Textmining
acidocalcisome vesicular acidic compartment, VSP1 occurs in metacyclic forms Leishmania amazonensis 20022
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ VSP1 is dependent on divalent cations Mg2+ or Zn2+, Mg2+ is absolutely required for determination of substrate specificity and cannot be substituted by other metal ions Leishmania amazonensis
Zn2+ VSP1 is dependent on divalent cations Mg2+ or Zn2+ Leishmania amazonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Leishmania amazonensis
-
 2 phosphate
-
 ?
diphosphate + H2O Leishmania amazonensis MHOM/BR/1987/BA125
-
 2 phosphate
-
 ?
additional information Leishmania amazonensis VSP1 is not essential for Leishmania promastigote growth and macrophage infection but for metacyclogenesis, VSP1 is essential to maintain virulence in mice ?
-
 ?
additional information Leishmania amazonensis MHOM/BR/1987/BA125 VSP1 is not essential for Leishmania promastigote growth and macrophage infection but for metacyclogenesis, VSP1 is essential to maintain virulence in mice ?
-
 ?
polyphosphate-n28 + H2O Leishmania amazonensis
-
 ?
-
 ?
polyphosphate-n28 + H2O Leishmania amazonensis MHOM/BR/1987/BA125
-
 ?
-
 ?
triphosphate + H2O Leishmania amazonensis
-
 3 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania amazonensis Q7Z031 MHOM/BR/1987/BA125
-
Leishmania amazonensis MHOM/BR/1987/BA125 Q7Z031 MHOM/BR/1987/BA125
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Leishmania amazonensis

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression analysis Leishmania amazonensis
-
promastigote
-
 Leishmania amazonensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Leishmania amazonensis 2 phosphate
-
 ?
diphosphate + H2O Mg2+ is absolutely required for determination of substrate specificity Leishmania amazonensis 2 phosphate
-
 ?
diphosphate + H2O
-
 Leishmania amazonensis MHOM/BR/1987/BA125 2 phosphate
-
 ?
diphosphate + H2O Mg2+ is absolutely required for determination of substrate specificity Leishmania amazonensis MHOM/BR/1987/BA125 2 phosphate
-
 ?
additional information VSP1 is not essential for Leishmania promastigote growth and macrophage infection but for metacyclogenesis, VSP1 is essential to maintain virulence in mice Leishmania amazonensis ?
-
 ?
additional information VSP1 is not essential for Leishmania promastigote growth and macrophage infection but for metacyclogenesis, VSP1 is essential to maintain virulence in mice Leishmania amazonensis MHOM/BR/1987/BA125 ?
-
 ?
polyphosphate-n28 + H2O
-
 Leishmania amazonensis ?
-
 ?
polyphosphate-n28 + H2O chain length of 28 phosphates Leishmania amazonensis ?
-
 ?
polyphosphate-n28 + H2O
-
 Leishmania amazonensis MHOM/BR/1987/BA125 ?
-
 ?
polyphosphate-n28 + H2O chain length of 28 phosphates Leishmania amazonensis MHOM/BR/1987/BA125 ?
-
 ?
triphosphate + H2O
-
 Leishmania amazonensis 3 phosphate
-
 ?

Subunits

Subunits Comment Organism
More VSP1 is preferably expressed in metacyclic forms Leishmania amazonensis

Synonyms

Synonyms Comment Organism
acidocalcisomal pyrophosphatase
-
 Leishmania amazonensis
soluble pyrophosphatase
-
 Leishmania amazonensis
VSP1
-
 Leishmania amazonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Leishmania amazonensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 polyphosphate-n28 25°C Leishmania amazonensis
125
-
 diphosphate 25°C Leishmania amazonensis
131
-
 Triphosphate 25°C Leishmania amazonensis