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Literature summary for 7.1.3.1 extracted from

  • Drozdowicz, Y.M.; Shaw, M.; Nishi, M.; Striepen, B.; Liwinski, H.A.; Roos, D.S.; Rea, P.A.
    Isolation and characterization of TgVP1, a type I vacuolar H+-translocating pyrophosphatase from Toxoplasma gondii. The dynamics of subcellular localization and the cellular effects of a diphosphonate inhibitor (2003), J. Biol. Chem., 278, 1075-1085.
    View publication on PubMed
Search for more literature for 7.1.3.1

Application

Application Comment Organism
medicine enzyme might serve as effective drug target Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae BJ5459 Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
aminomethylenediphosphonate IC50: 0.0009 mM Toxoplasma gondii
Ca2+ very potent inhibitor Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 Toxoplasma gondii 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 2-3fold activation Toxoplasma gondii
Mg2+ required Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Toxoplasma gondii
85000
-
 calculated from predicted amino acid sequence Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Toxoplasma gondii important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] Toxoplasma gondii RH important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q9BK08 cDNA
-
Toxoplasma gondii RH Q9BK08 cDNA
-

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Toxoplasma gondii 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Toxoplasma gondii 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1]
-
 Toxoplasma gondii RH 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Toxoplasma gondii RH 2 phosphate + H+[side 2]
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0009
-
 IC50: 0.0009 mM Toxoplasma gondii aminomethylenediphosphonate