Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates activity | Trypanosoma cruzi | |
K+ | stimulates activity | Plasmodium falciparum | |
K+ | stimulates activity | Thermotoga maritima | |
K+ | stimulates activity | Toxoplasma gondii | |
additional information | insensitive to stimulation with K+ | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E263D | 50% of hydrolytic activity, no protein translocation | Vigna radiata |
K261R | 25% of hydrolytic activity, no protein translocation | Vigna radiata |
V259A | 60% of hydrolytic activity, no protein translocation | Vigna radiata |
V262A | no effect on activity | Vigna radiata |
General Stability | Organism |
---|---|
Mg2+ protects against inactivation and protease digestion | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bisphosphonates | weak inhibition | Plasmodium falciparum | |
bisphosphonates | - |
Toxoplasma gondii | |
bisphosphonates | - |
Trypanosoma cruzi | |
risedronate | IC50: 0.123 mM | Plasmodium falciparum | |
risedronate | IC50: 0.002 mM | Toxoplasma gondii | |
risedronate | IC50: 0.123 mM | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
acidocalcisome | - |
Trypanosoma cruzi | 20022 | - |
membrane | - |
Plasmodium falciparum | 16020 | - |
membrane | - |
Rhodospirillum rubrum | 16020 | - |
membrane | - |
Thermotoga maritima | 16020 | - |
membrane | - |
Pyrobaculum aerophilum | 16020 | - |
plasma membrane | - |
Trypanosoma cruzi | 5886 | - |
plasma membrane | - |
Toxoplasma gondii | 5886 | - |
vacuolar membrane | - |
Zea mays | 5774 | - |
vacuolar membrane | - |
Arabidopsis thaliana | 5774 | - |
vacuolar membrane | - |
Vigna radiata | 5774 | - |
vacuolar membrane | - |
Toxoplasma gondii | 5774 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Zea mays | |
Mg2+ | required | Arabidopsis thaliana | |
Mg2+ | required | Trypanosoma cruzi | |
Mg2+ | required | Vigna radiata | |
Mg2+ | required | Plasmodium falciparum | |
Mg2+ | required | Rhodospirillum rubrum | |
Mg2+ | required | Thermotoga maritima | |
Mg2+ | required | Pyrobaculum aerophilum | |
Mg2+ | required | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Zea mays | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Arabidopsis thaliana | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Trypanosoma cruzi | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Vigna radiata | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Plasmodium falciparum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Rhodospirillum rubrum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Thermotoga maritima | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Pyrobaculum aerophilum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Toxoplasma gondii | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Plasmodium falciparum | - |
hyperthermophilic bacterium | - |
Pyrobaculum aerophilum | - |
hyperthermophilic bacterium | - |
Rhodospirillum rubrum | - |
- |
- |
Thermotoga maritima | - |
hyperthermophilic bacterium | - |
Toxoplasma gondii | - |
- |
- |
Trypanosoma cruzi | - |
hyperthermophilic bacterium | - |
Vigna radiata | - |
mung bean | - |
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Zea mays | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Arabidopsis thaliana | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Trypanosoma cruzi | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Plasmodium falciparum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
r | |
diphosphate + H2O + H+[side 1] | - |
Thermotoga maritima | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Pyrobaculum aerophilum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Toxoplasma gondii | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Zea mays | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Arabidopsis thaliana | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Trypanosoma cruzi | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Plasmodium falciparum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Thermotoga maritima | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Pyrobaculum aerophilum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Toxoplasma gondii | 2 phosphate + H+[side 2] | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
IC50: 0.002 mM | Toxoplasma gondii | risedronate | |
0.123 | - |
IC50: 0.123 mM | Trypanosoma cruzi | risedronate | |
0.123 | - |
IC50: 0.123 mM | Plasmodium falciparum | risedronate |