Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOP10F' | Ascaris suum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Ascaris suum | |
Imidodiphosphate | slight inhibition | Ascaris suum | |
NaF | - |
Ascaris suum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.117 | - |
diphosphate | recombinant protein, pH 7.5, 55°C | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II enzyme, proton translocating form | Ascaris suum | 16020 | - |
soluble | type I enzyme | Ascaris suum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Ascaris lumbricoides | |
Mg2+ | required | Toxocara canis | |
Mg2+ | required, highest activity at 5 mM, decrease of activity at higher concentrations | Ascaris suum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38770 | - |
calculated from the predicted amino acid sequence after removal of the signal peptide | Ascaris suum |
39000 | - |
SDS-PAGE | Ascaris suum |
40600 | - |
calculated from predicted amino acid sequence of the entire ORF | Ascaris suum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Ascaris lumbricoides | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Ascaris suum | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | Toxocara canis | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | 2 phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris lumbricoides | - |
- |
- |
Ascaris suum | Q86M43 | soluble enzyme | - |
Toxocara canis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Ascaris suum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall | - |
Ascaris suum | - |
gut epithelium | - |
Ascaris suum | - |
ovary | - |
Ascaris suum | - |
uterus | - |
Ascaris suum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
937 | - |
recombinant protein, pH 7.5, 55°C | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Ascaris lumbricoides | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Ascaris suum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | - |
Toxocara canis | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | Ascaris lumbricoides | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | Ascaris suum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation | Toxocara canis | 2 phosphate + H+[side 2] | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
638 | - |
diphosphate | recombinant protein, pH 7.5, 55°C | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Ascaris suum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | - |
Ascaris suum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ascaris suum | calculated from predicted amino acid sequence and detected in isoelectric focusing | - |
7.1 |