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Literature summary for 7.1.3.1 extracted from
- Purification, physicochemical properties, and subcellular location of alkaline inorganic pyrophosphatase from sesame (Sesamum indicum L.) cotyledons (2002), Biochem. Cell Biol., 80, 215-224.
General Stability
| General Stability | Organism |
|---|---|
| 4 M urea for 30 min does not affect activity | Sesamum indicum |
| Mg2+ stabilizes enzyme | Sesamum indicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Sesamum indicum |  |
| Ca2+ | - |
Sesamum indicum | |
| Cu2+ | - |
Sesamum indicum | |
| Fe3+ | - |
Sesamum indicum | |
| Hg2+ | - |
Sesamum indicum | |
| Zn2+ | - |
Sesamum indicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.768 | - |
diphosphate | pH 8.6, 37°C | Sesamum indicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glyoxysome | 63% of activity | Sesamum indicum | 9514 | - |
| mitochondrion | 23% of activity | Sesamum indicum | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight activation | Sesamum indicum | |
| Mg2+ | required, highest activity with a Mg2+/diphosphate ratio of 2 | Sesamum indicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20100 | - |
SDS-PAGE | Sesamum indicum |
| 20800 | - |
gel filtration | Sesamum indicum |
| 21900 | - |
calculated from amino acid sequence | Sesamum indicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O + H+[side 1] | Sesamum indicum | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate + H+[side 2] | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 70% of activity in 50% solution | Sesamum indicum |
| Methanol | 70% of activity in 50% solution | Sesamum indicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sesamum indicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sesamum indicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Sesamum indicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.66 | - |
purified enzyme, pH 8.6, 37°C | Sesamum indicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O + H+[side 1] | - |
Sesamum indicum | 2 phosphate + H+[side 2] | - |
? | |
| diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions | Sesamum indicum | 2 phosphate + H+[side 2] | - |
? | |
| additional information | trimetaphosphate and tripolyphosphate are hydrolyzed with 2% of the rate with diphosphate, no other phosphorylated compounds are hydrolyzed | Sesamum indicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | same molecular weight in SDS-PAGE and gel filtration | Sesamum indicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline PPase | - |
Sesamum indicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sesamum indicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | - |
Sesamum indicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for up to 30 min | Sesamum indicum |
| 60 | - |
33% loss of activity within 30 min | Sesamum indicum |
| 80 | - |
complete loss of activity within 15 min | Sesamum indicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Sesamum indicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | - |
Sesamum indicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable | Sesamum indicum |
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