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Literature summary for 7.1.3.1 extracted from
- H+-translocating inorganic pyrophosphatase of plant vacuoles. Inhibition by Ca2+, stabilization by Mg2+ and immunological comparison with other inorganic pyrophosphatases (1991), Eur. J. Biochem., 196, 11-17.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strong, potent | Vigna radiata |  |
| CaCl2 | inhibitory form is the Ca2+diphosphate complex | Vigna radiata | |
| Cd2+ | - |
Vigna radiata | |
| Co2+ | - |
Vigna radiata | |
| Cu2+ | - |
Vigna radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
diphosphate | - |
Vigna radiata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| tonoplast | - |
Vigna radiata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity and stability | Vigna radiata | |
| Mg2+ | required for activity | Vigna radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O + H+[side 1] | Vigna radiata | actual substrate is magnesium diphosphate or dimagnesium diphosphate | 2 phosphate + H+[side 2] | - |
? | |
| diphosphate + H2O + H+[side 1] | Vigna radiata | H+-pump | 2 phosphate + H+[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypocotyl | - |
Vigna radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O + H+[side 1] | actual substrate is magnesium diphosphate or dimagnesium diphosphate | Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
| diphosphate + H2O + H+[side 1] | H+-pump | Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
| diphosphate + H2O + H+[side 1] | diphosphate in form of Mg-diphosphate | Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
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