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Literature summary for 7.1.3.1 extracted from

  • Wang, Y.; Leigh, R.A.; Kaestner, K.H.; Sze, H.
    Electrogenic H+-pumping pyrophosphatase in tonoplast vesicles of oat roots (1986), Plant Physiol., 81, 497-502.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
 Avena sativa
F- IC50: 1-2 mM Avena sativa
Li+
-
 Avena sativa
N-ethylmaleimide
-
 Avena sativa
Na+
-
 Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 diphosphate
-
 Avena sativa
4
-
 K+
-
 Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
tonoplast
-
 Avena sativa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for activity Avena sativa
Mg2+
-
 Avena sativa
Rb+
-
 Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Avena sativa H+-pump 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 oat
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Avena sativa 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] H+-pump Avena sativa 2 phosphate + H+[side 2]
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 3-4 h, 50% loss of activity Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Avena sativa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1 2 IC50: 1-2 mM Avena sativa F-