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Literature summary for 7.1.2.2 extracted from

  • Xu, T.; Forgac, M.
    Subunit D (Vma8p) of the yeast vacuolar H+-ATPase plays a role in coupling of proton transport and ATP hydrolysis (2000), J. Biol. Chem., 275, 22075-22081 .
    View publication on PubMed
Search for more literature for 7.1.2.2

Protein Variants

Protein Variants Comment Organism
D218V the mutant shows 85% of wild type ATPase activity Saccharomyces cerevisiae
D249G the mutant shows 64% of wild type ATPase activity Saccharomyces cerevisiae
E220V the mutant shows 73% of wild type ATPase activity Saccharomyces cerevisiae
G80D the mutant shows 90% of wild type ATPase activity Saccharomyces cerevisiae
G80D/E220V the mutant shows 50% of wild type ATPase activity Saccharomyces cerevisiae
G80D/E220V/M221V the mutant shows 10% of wild type ATPase activity Saccharomyces cerevisiae
G80D/K209E the double mutant shows increased coupling efficiency of proton transport and ATPase activity Saccharomyces cerevisiae
I188N the mutant shows 62% of wild type ATPase activity Saccharomyces cerevisiae
I188N/I173N/A232T the mutant shows 20% of wild type ATPase activity Saccharomyces cerevisiae
I188N/R198G the mutant shows 15% of wild type ATPase activity Saccharomyces cerevisiae
K209E the mutant shows 110% of wild type ATPase activity Saccharomyces cerevisiae
K210E the mutant shows 95% of wild type ATPase activity Saccharomyces cerevisiae
K210E/D218V the mutant shows 42% of wild type ATPase activity Saccharomyces cerevisiae
L149V the mutant shows wild type ATPase activity Saccharomyces cerevisiae
L149V/D249G the mutant shows 55% of wild type ATPase activity Saccharomyces cerevisiae
L149V/E182D/D249G the mutant shows 35% of wild type ATPase activity Saccharomyces cerevisiae
M221V the mutant shows 50% of wild type ATPase activity Saccharomyces cerevisiae
M221V the mutation leads to significant uncoupling of proton transport and ATPase activity Saccharomyces cerevisiae
N100I the mutant shows 60% of wild type ATPase activity Saccharomyces cerevisiae
P179S the mutant shows 60% of wild type ATPase activity Saccharomyces cerevisiae
R198G the mutant shows 96% of wild type ATPase activity Saccharomyces cerevisiae
V104E the mutant shows wild type ATPase activity Saccharomyces cerevisiae
V71D the mutant shows 30% of wild type ATPase activity Saccharomyces cerevisiae
V71D/E220V the mutant shows 20% of wild type ATPase activity Saccharomyces cerevisiae
V71D/E220V the mutations lead to significant uncoupling of proton transport and ATPase activity Saccharomyces cerevisiae
V71D/E220V/M221V the mutant shows 7% of wild type ATPase activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+[side 1] Saccharomyces cerevisiae
-
 ADP + phosphate + H+[side 2]
-
 ?
ATP + H2O + H+[side 1] Saccharomyces cerevisiae KHY105
-
 ADP + phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae KHY105
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + H+[side 2]
-
 ?
ATP + H2O + H+[side 1]
-
 Saccharomyces cerevisiae KHY105 ADP + phosphate + H+[side 2]
-
 ?
additional information subunit D plays an important role in coupling of proton transport and ATP hydrolysis Saccharomyces cerevisiae ?
-
 ?
additional information subunit D plays an important role in coupling of proton transport and ATP hydrolysis Saccharomyces cerevisiae KHY105 ?
-
 ?

Synonyms

Synonyms Comment Organism
V-ATPase
-
 Saccharomyces cerevisiae
vacuolar H+-ATPase
-
 Saccharomyces cerevisiae
VMA8 gene name, encoding subunit D Saccharomyces cerevisiae
Vma8p subunit D Saccharomyces cerevisiae