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Literature summary for 7.1.2.2 extracted from

  • Samra, H.S.; He, F.; Degner, N.R.; Richter, M.L.
    The role of specific beta-gamma subunit interactions in oxyanion stimulation of the MgATP hydrolysis of a hybrid photosynthetic F1-ATPase (2008), J. Bioenerg. Biomembr., 40, 69-76.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K30C/A276C alpha3beta3gamma, the mutant shows assembly as the wild-type enzyme complex Rhodospirillum rubrum
K30C/R278C alpha3beta3gamma, the mutant shows assembly as the wild-type enzyme complex Rhodospirillum rubrum
additional information pairs of cysteine residues were introduced into the twisted N- and C-terminal helices of the gamma subunit of the chloroplast F1-ATPase to test, via disulfide cross-linking, potential inter-helical movements involved in catalysis of ATP hydrolysis. Significant disulfide formation of 50-75% is observed between cysteines introduced at positions 30 and 31 in the N-terminal helix and 276 and 278 in the C-terminal helix, cross-linking has no apparent effect on catalysis Rhodospirillum rubrum
V31C/A276C alpha3beta3gamma, the mutant shows assembly as the wild-type enzyme complex Rhodospirillum rubrum
V31C/R278C alpha3beta3gamma, the mutant shows assembly as the wild-type enzyme complex Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Rhodospirillum rubrum 9507
-
membrane
-
Rhodospirillum rubrum 16020
-

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Subunits

Subunits Comment Organism
multimer the ATP synthase enzymes of chloroplasts is composed of two protein segments, FO and F1, the chloroplast FO1 contains four different polypeptide subunits with a stoichiometry of I1II1III14IV1. The F1 segment contains the catalytic sites for ATP synthesis and hydrolysis. The chloroplast F1 is comprised of five different polypeptide subunits, alpha to epsilon, with a stoichiometry of alpha3beta3gamma1delta1epsilon1 Rhodospirillum rubrum

Synonyms

Synonyms Comment Organism
photosynthetic F1-ATPase
-
Rhodospirillum rubrum