Literature summary for 7.1.2.2 extracted from

Bowler, M.W.; Montgomery, M.G.; Leslie, A.G.; Walker, J.E.
Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F(1)-ATPase by controlled dehydration (2006), Acta Crystallogr. Sect. D, 62, 991-995.

Crystallization (Commentary)

Crystallization (Comment)	Organism
orthorhombic crystals of F1-ATPase, grown during 4 weeks from 5 mg/ml protein solution mixed with an equal volume of crystallization solution containing 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 20 mM MgSO4, 0.02% w/v NaN3, 0.004% w/v PMSF, 1 mM ATP and 9-12% w/v PEG 6000 in D2O, are subjected to controlled dehydration resulting in a decrease in relative humidity surrounding the crystals to 90%, in reduced unit-cell volume by 22%, and significantly improved diffraction limit and mosaic spread of the crystals, X-ray diffraction and structure analysis at 1.8 A resolution, radiation damage limits the resolution of a complete data set to 1.95 A, overview	Bos taurus

Crystallization (Comment)

Organism

orthorhombic crystals of F1-ATPase, grown during 4 weeks from 5 mg/ml protein solution mixed with an equal volume of crystallization solution containing 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 20 mM MgSO4, 0.02% w/v NaN3, 0.004% w/v PMSF, 1 mM ATP and 9-12% w/v PEG 6000 in D2O, are subjected to controlled dehydration resulting in a decrease in relative humidity surrounding the crystals to 90%, in reduced unit-cell volume by 22%, and significantly improved diffraction limit and mosaic spread of the crystals, X-ray diffraction and structure analysis at 1.8 A resolution, radiation damage limits the resolution of a complete data set to 1.95 A, overview

Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial membrane	-	Bos taurus	31966	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + H+/in	Bos taurus	F1Fo-ATPase is a large membrane-bound multisubunit complex that catalyses the synthesis of ATP from ADP and phosphate using a transmembrane proton motive force generated by respiration or photosynthesis as a source of energy, ATP hydrolytic catalysis takes place in its hydrophilic F1 domain	ADP + phosphate + H+/out	-	ir

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Bos taurus	ADP + phosphate + H+/out	-	?
ATP + H2O + H+/in	F1Fo-ATPase is a large membrane-bound multisubunit complex that catalyses the synthesis of ATP from ADP and phosphate using a transmembrane proton motive force generated by respiration or photosynthesis as a source of energy, ATP hydrolytic catalysis takes place in its hydrophilic F1 domain	Bos taurus	ADP + phosphate + H+/out	-	ir

Synonyms

Synonyms	Comment	Organism
mitochondrial F(1)-ATPase	-	Bos taurus