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Literature summary for 7.1.2.1 extracted from

  • Petrov, V.; Ibragimov, R.
    Effect of point substitutions of Asp-714 and Asp-720 residues on the structure and function of the H+-ATPase of the yeast plasma membrane (2014), Appl. Biochem. Microbiol., 50, 508-515.
    View publication on PubMed
Search for more literature for 7.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D714A 22% of wild-type expression, intracellular trafficking is disrupted Saccharomyces cerevisiae
D714E 12% of wild-type expression, intracellular trafficking is disrupted Saccharomyces cerevisiae
D714N 67% of wild-type expression Saccharomyces cerevisiae
D714V 4% of wild-type expression, intracellular trafficking is disrupted Saccharomyces cerevisiae
D720A 63% of wild-type expression Saccharomyces cerevisiae
D720E 61% of wild-type expression Saccharomyces cerevisiae
D720N 72% of wild-type expression Saccharomyces cerevisiae
D720V 41% of wild-type expression Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
vanadate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 ATP mutant D720A, pH 5.7, 30°C Saccharomyces cerevisiae
0.7
-
 ATP mutant D720E, pH 5.7, 30°C Saccharomyces cerevisiae
0.9
-
 ATP mutant D714N, pH 5.7, 30°C Saccharomyces cerevisiae
0.9
-
 ATP mutant D720N, pH 5.7, 30°C Saccharomyces cerevisiae
1
-
 ATP mutant D720V, pH 5.7, 30°C Saccharomyces cerevisiae
1.3
-
 ATP wild-type, pH 5.7, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae SY4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae SY4 ADP + phosphate + H+/out
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
 vanadate mutant D720V, pH 5.7, 30°C Saccharomyces cerevisiae
0.0027
-
 vanadate wild-type, pH 5.7, 30°C Saccharomyces cerevisiae
0.0033
-
 vanadate mutant D714N, pH 5.7, 30°C Saccharomyces cerevisiae
0.0035
-
 vanadate mutant D720E, pH 5.7, 30°C Saccharomyces cerevisiae
0.0037
-
 vanadate mutant D720N, pH 5.7, 30°C Saccharomyces cerevisiae
0.0043
-
 vanadate mutant D720A, pH 5.7, 30°C Saccharomyces cerevisiae