Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae NY13 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E803A | 15% ATPase and 13% H+ transport activity compared to the wild type enzyme. The mutation has no significant influence on the ATPase and cell sensitivity to heat shock. However, it causes a shift in the equilibrium between E1 and E2 conformations of the enzyme towards E1 | Saccharomyces cerevisiae |
F796A | 4% ATPase and no H+ transport activity compared to the wild type enzyme. The mutation causes enzyme and cell sensitivity to heat shock when expressed in secretory vesicles | Saccharomyces cerevisiae |
I794A | 4% ATPase and no H+ transport activity compared to the wild type enzyme. The mutation increases temperature sensitivity of cells when the enzyme is expressed either in secretory vesicles or, to a lesser extent, in plasma membrane | Saccharomyces cerevisiae |
I799A | 2% ATPase and no H+ transport activity compared to the wild type enzyme. The mutation is lethal for cells regardless of expression of the enzyme in secretory vesicles or plasma membrane | Saccharomyces cerevisiae |
I807A | 11% ATPase and no H+ transport activity compared to the wild type enzyme | Saccharomyces cerevisiae |
L797A | 20% ATPase and 17% H+ transport activity compared to the wild type enzyme | Saccharomyces cerevisiae |
L801A | 7% ATPase and no H+ transport activity compared to the wild type enzyme | Saccharomyces cerevisiae |
Q798A | 1% ATPase and no H+ transport activity compared to the wild type enzyme. The mutation is lethal for cells regardless of expression of the enzyme in secretory vesicles or plasma membrane | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
orthovanadate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | mutant enzyme E803A, at pH 5.7 and 30°C | Saccharomyces cerevisiae | |
0.7 | - |
ATP | mutant enzyme L797A, at pH 5.7 and 30°C | Saccharomyces cerevisiae | |
1.4 | - |
ATP | wild type enzyme, at pH 5.7 and 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
secretory vesicle | - |
Saccharomyces cerevisiae | 99503 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae SY4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Saccharomyces cerevisiae | ADP + phosphate + H+/out | - |
? | |
ATP + H2O + H+/in | - |
Saccharomyces cerevisiae SY4 | ADP + phosphate + H+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H+-ATPase | - |
Saccharomyces cerevisiae |
PMA1 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | H+-ATPase is a key enzyme of cell metabolism generating electrochemical proton gradient across the plasma membrane, thus playing an important role in the maintenance of ion homeostasis in the cell | Saccharomyces cerevisiae |