Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.2.1 extracted from

  • Ottmann, C.; Marco, S.; Jaspert, N.; Marcon, C.; Schauer, N.; Weyand, M.; Vandermeeren, C.; Duby, G.; Boutry, M.; Wittinghofer, A.; Rigaud, J.L.; Oecking, C.
    Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+ -ATPase by combining X-ray crystallography and electron cryomicroscopy (2007), Mol. Cell, 25, 427-440.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Nicotiana plumbaginifolia

Crystallization (Commentary)

Crystallization (Comment) Organism
regulatory protein 14-3-3 in complex with the entire binding motif of the enzyme, i.e. residues 905-956 expressed in yeast, and in complex with the hexameric enzyme. Three 14-3-3 dimers are located on top of a PMA2 hexamer where they may coordinate six autoinhibitory domains of the active enzyme Nicotiana plumbaginifolia

Protein Variants

Protein Variants Comment Organism
H930A binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is significantly reduced Nicotiana plumbaginifolia
K943E binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is significantly reduced Nicotiana plumbaginifolia
L932A binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is significantly reduced Nicotiana plumbaginifolia
S938A binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is increased Nicotiana plumbaginifolia
S938E binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is significantly reduced Nicotiana plumbaginifolia
T931A binding of regulatory protein 14-3-3 to the entire binding motif of the enzyme, i.e. residues 905-956 bearing the mutation, is significantly reduced Nicotiana plumbaginifolia

Organism

Organism UniProt Comment Textmining
Nicotiana plumbaginifolia
-
-
-

Synonyms

Synonyms Comment Organism
PMA2
-
Nicotiana plumbaginifolia