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Literature summary for 7.1.2.1 extracted from

  • Guerra, G.; Petrov, V.V.; Allen, K.E.; Miranda, M.; Pardo, J.P.; Slayman, C.W.
    Role of transmembrane segment M8 in the biogenesis and function of yeast plasma-membrane H(+)-ATPase (2007), Biochim. Biophys. Acta, 1768, 2383-2392.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.1

Protein Variants

Protein Variants Comment Organism
E803A 74% of wild-type activity Saccharomyces cerevisiae
F796A no catalytic activity Saccharomyces cerevisiae
F808A 83% of wild-type activity Saccharomyces cerevisiae
G793A 38% of wild-type activity Saccharomyces cerevisiae
G793E 36% of wild-type activity Saccharomyces cerevisiae
I794A no catalytic activity Saccharomyces cerevisiae
I799A no catalytic activity Saccharomyces cerevisiae
I807A no catalytic activity Saccharomyces cerevisiae
I809A 88% of wild-type activity Saccharomyces cerevisiae
L797A 105% of wild-type activity Saccharomyces cerevisiae
L801A no catalytic activity Saccharomyces cerevisiae
L806A 120% of wild-type activity Saccharomyces cerevisiae
M791A 49% of wild-type activity Saccharomyces cerevisiae
M795A 45% of wild-type activity Saccharomyces cerevisiae
N792A 53% of wild-type activity Saccharomyces cerevisiae
N792D 98% of wild-type activity Saccharomyces cerevisiae
N792H 64% of wild-type activity Saccharomyces cerevisiae
N792Q 118% of wild-type activity Saccharomyces cerevisiae
N804A 46% of wild-type activity Saccharomyces cerevisiae
Q798A no catalytic activity Saccharomyces cerevisiae
Q798E 78% of wild-type activity Saccharomyces cerevisiae
R811A 86% of wild-type activity Saccharomyces cerevisiae
S800A 89% of wild-type activity, mutation in the middle of transmembrane helix M8, increase in apparent stoichiometry of H+ transport Saccharomyces cerevisiae
T802A 52% of wild-type activity Saccharomyces cerevisiae
T810A 84% of wild-type activity Saccharomyces cerevisiae
W805A 65% of wild-type activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
vanadate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 ATP mutant M795A, pH 5.7, 30°C Saccharomyces cerevisiae
0.7
-
 ATP mutant E803A, pH 5.7, 30°C Saccharomyces cerevisiae
0.7
-
 ATP mutant L797A, pH 5.7, 30°C Saccharomyces cerevisiae
0.8
-
 ATP mutant F808A, pH 5.7, 30°C Saccharomyces cerevisiae
0.8
-
 ATP mutant G793E, pH 5.7, 30°C Saccharomyces cerevisiae
0.9
-
 ATP mutant R811A, pH 5.7, 30°C Saccharomyces cerevisiae
0.9
-
 ATP mutant W805A, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant G793A, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant N792A, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant N792H, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant Q798E, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant T802A, pH 5.7, 30°C Saccharomyces cerevisiae
1.1
-
 ATP mutant T810A, pH 5.7, 30°C Saccharomyces cerevisiae
1.2
-
 ATP mutant N792Q, pH 5.7, 30°C Saccharomyces cerevisiae
1.3
-
 ATP mutant M791A, pH 5.7, 30°C Saccharomyces cerevisiae
1.3
-
 ATP mutant N792D, pH 5.7, 30°C Saccharomyces cerevisiae
1.4
-
 ATP mutant S800A, pH 5.7, 30°C Saccharomyces cerevisiae
1.4
-
 ATP wild-type, pH 5.7, 30°C Saccharomyces cerevisiae
1.5
-
 ATP mutant I809A, pH 5.7, 30°C Saccharomyces cerevisiae
1.6
-
 ATP mutant N804A, pH 5.7, 30°C Saccharomyces cerevisiae
1.8
-
 ATP mutant L806A, pH 5.7, 30°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Saccharomyces cerevisiae 5886
-
secretory vesicle
-
 Saccharomyces cerevisiae 99503
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P05030
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae ADP + phosphate + H+/out
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 mutants G793A, G793E Saccharomyces cerevisiae
5.6
-
 mutant L797A Saccharomyces cerevisiae
5.7
-
 wild-type, mutants M791A, M795A, S800A, T802A, E803A, N804A, W805A, L806A, F808A, I809A, T810A, R811A, N792D, N792Q, N792H, Q798E Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0013
-
 mutant N792D, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0015
-
 mutant M791A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0015
-
 mutant N792H, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0015
-
 mutant N792Q, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0015
-
 mutant Q798E, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0015
-
 wild-type, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0018
-
 mutant I809A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0018
-
 mutant L797A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0018
-
 mutant T802A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0019
-
 mutant M795A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.002
-
 mutant G793E, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.002
-
 mutant L806A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0021
-
 mutant S800A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0021
-
 mutant T810A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0022
-
 mutant N792A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0022
-
 mutant R811A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0022
-
 mutant W805A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0026
-
 mutant F808A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0027
-
 mutant N804A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0029
-
 mutant G793A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate
0.0042
-
 mutant E803A, pH 5.7, 30°C Saccharomyces cerevisiae vanadate