Literature summary for 7.1.1.9 extracted from

Trubitsina, L.I.; Tishchenko, S.V.; Gabdulkhakov, A.G.; Lisov, A.V.; Zakharova, M.V.; Leontievsky, A.A.
Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes (2015), Biochimie, 112, 151-159 .

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Cloned(Commentary)

Cloned (Comment)	Organism
overproduced in Escherichia coli	Streptomyces viridochromogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion method, X-ray quality crystals are generated and the structure is solved to a resolution of 2.4 A	Streptomyces viridochromogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Streptomyces viridochromogenes
additional information	the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF	Streptomyces viridochromogenes

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	the recombinant protein is found in the cytoplasmic fraction	Streptomyces viridochromogenes	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	copper-containing enzyme. Contains 4 atoms per molecule of the enzyme	Streptomyces viridochromogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88000	90000	gel filtration	Streptomyces viridochromogenes

Organism

Organism	UniProt	Comment	Textmining
Streptomyces viridochromogenes	J9S5G3	-	-
Streptomyces viridochromogenes VKM Ac-629	J9S5G3	-	-

Storage Stability

Storage Stability	Organism
20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity	Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4 ferrocytochrome c + O2 + 4 H+	-	Streptomyces viridochromogenes	4 ferricytochrome c + 2 H2O	-	?
4 ferrocytochrome c + O2 + 4 H+	-	Streptomyces viridochromogenes VKM Ac-629	4 ferricytochrome c + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 33900, calculated from sequence	Streptomyces viridochromogenes
homotrimer	3 * 39000, SDS-PAGE after boiling	Streptomyces viridochromogenes

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2	-	20°C, 2 days, the enzyme retains about 30% residual activity	Streptomyces viridochromogenes
3	-	20°C, 2 days, the enzyme retains about 42% residual activity	Streptomyces viridochromogenes
4	-	20°C, 2 days, the enzyme retains about 65-60% residual activity	Streptomyces viridochromogenes
5	-	20°C, 2 days, the enzyme retains about 65-60% residual activity	Streptomyces viridochromogenes
6	10	20°C, 2 days, the enzyme retains about 70% residual activity	Streptomyces viridochromogenes

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Release 2023.1 (January 2023)