Literature summary for 7.1.1.9 extracted from

Rigby, K.; Zhang, L.; Cobine, P.A.; George, G.N.; Winge, D.R.
characterization of the cytochrome c oxidase assembly factor Cox19 of Saccharomyces cerevisiae (2007), J. Biol. Chem., 282, 10233-10242.

Search for more literature for 7.1.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant Cox19 expressed in Escherichia coli BL21 (pLysS) as a fusion protein with an 8-residue C-terminal Strep-tag II extension	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
C30A	is growth-compromised on glycerol/lactate. Is compromised in Cu(I) binding	Saccharomyces cerevisiae
C30A/C62A	is growth-compromised. Is more severely compromised than the single C30A mutant. Fails to accumulate in mitochondria. Is compromised in Cu(I) binding	Saccharomyces cerevisiae
C40A/C52A	is functional	Saccharomyces cerevisiae
H26A	is able to propagate on glycerol/lactate medium	Saccharomyces cerevisiae
H26A/C30A	is growth-compromised. Fails to accumulate in mitochondria. Is compromised in Cu(I) binding	Saccharomyces cerevisiae
additional information	CcO activity in cox19DLETA cells is less than 10% of wild-type levels	Saccharomyces cerevisiae
R63T	is nonfunctional even when expressed as an IM-tethered Cox19 fusion	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	intermembrane space	Saccharomyces cerevisiae	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	recombinant Cox19 binds 1 mol eq of Cu(I) per monomer. Cu(I) binding may be a transient property	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae BY4742	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by size exclusion chromatography	Saccharomyces cerevisiae

Subunits

Subunits	Comment	Organism
dimer	CuCox19 and apoCox19, sedimentation equilibrium	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
COX19	-	Saccharomyces cerevisiae
cytochrome c oxidase	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)