Literature summary for 7.1.1.9 extracted from

Qin, L.; Mills, D.A.; Hiser, C.; Murphree, A.; Garavito, R.M.; Ferguson-Miller, S.; Hosler, J.
Crystallographic location and mutational analysis of Zn and Cd inhibitory sites and role of lipidic carboxylates in rescuing proton path mutants in cytochrome c oxidase (2007), Biochemistry, 46, 6239-6248.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	arachidonic acid, cholic acid and CHAPS have no effect on the activity of wild-type CcO	Cereibacter sphaeroides

Crystallization (Commentary)

Crystallization (Comment)	Organism
by sitting drop vapor diffusion method	Cereibacter sphaeroides

Protein Variants

Protein Variants	Comment	Organism
E101A	exhibits low activity and eliminates metal binding at this site. Significant activity is restored by adding lipophilic carboxylic compounds (arachidonic acid and cholic acid), but not by their non-carboxylic analogues	Cereibacter sphaeroides
E101A/H96A	exhibits low activity and eliminates metal binding at this site. Significant activity is restored by adding lipophilic carboxylic compounds (arachidonic acid and cholic acid), but not by their non-carboxylic analogues. Activity is still inhibited by zinc, but this remaining inhibition is nearly eliminated by removal of subunit III	Cereibacter sphaeroides

Inhibitors

Inhibitors	Comment	Organism	Structure
cadmium	-	Cereibacter sphaeroides
Zn2+	the Glu-101/His-96 site of subunit II as the site of metal binding inhibits the uptake of protons into the K pathway. Subunit III contributes to zinc binding and/or inhibition of the D pathway	Cereibacter sphaeroides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Cereibacter sphaeroides	16020	-

Organism

Organism	UniProt	Comment	Textmining
Cereibacter sphaeroides	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni-NTA column	Cereibacter sphaeroides

Synonyms

Synonyms	Comment	Organism
CCO	-	Cereibacter sphaeroides
COXII	-	Cereibacter sphaeroides
cytochrome c oxidase	-	Cereibacter sphaeroides

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Release 2023.1 (January 2023)