Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | overexpression of subunit Vb increases COX activity and decreases reactive oxygen species production in wild-type HeLa-20 cells, along with some increase in tolerance to hyperoxia | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
COX subunit Vb open reading frame ligated into the pcDNA3.1/V5-His TOPO-TA expression vector and transfected into TOP10 Escherichia coli. Overexpression in HeLa-20 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | small interfering RNA against Vb selectively lowers COX Vb expression in HeLa-80 cells, increases mitochondrial reactive oxygen species generation, decreases COX activity 60-80%, and diminishes viability under 80% (but not 20%) O2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa-80 cell | cells proliferate under 80% O2. Under both 20% and 80% O2, mitochondrial reactive oxygen species production is 2fold less in HeLa-80 cells than in wild-type HeLa cells, related to a 2fold higher cytochrome-c oxidase activity. Elevated enzyme activity is associated with a 2fold increase in the regulatory subunit Vb. Treatment with siRNA against subunit Vb lowers its expression, increases mitochondrial generation of reactive oxygen species, decreases enzyme activity by 60-80% and diminishes viability under 80% O2 | Homo sapiens | - |
HeLa-80 cell | elevated COX activity in the oxygen-tolerant HeLa-80 cells, associated with a more than 2fold increase in the regulatory subunit COX Vb, whereas expression levels of other subunits are very close to wild-type HeLa-20 cells | Homo sapiens | - |
additional information | HeLa-20 cell | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + O2 + H+ | - |
Homo sapiens | ferricytochrome c + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COX Vb | - |
Homo sapiens |
cytochrome c oxidase | - |
Homo sapiens |