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Literature summary for 7.1.1.9 extracted from

  • Campian, J.L.; Gao, X.; Qian, M.; Eaton, J.W.
    Cytochrome C oxidase activity and oxygen tolerance (2007), J. Biol. Chem., 282, 12430-12438.
    View publication on PubMed
Search for more literature for 7.1.1.9

Activating Compound

Activating Compound Comment Organism Structure
additional information overexpression of subunit Vb increases COX activity and decreases reactive oxygen species production in wild-type HeLa-20 cells, along with some increase in tolerance to hyperoxia Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
COX subunit Vb open reading frame ligated into the pcDNA3.1/V5-His TOPO-TA expression vector and transfected into TOP10 Escherichia coli. Overexpression in HeLa-20 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
siRNA small interfering RNA against Vb selectively lowers COX Vb expression in HeLa-80 cells, increases mitochondrial reactive oxygen species generation, decreases COX activity 60-80%, and diminishes viability under 80% (but not 20%) O2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
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Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa-80 cell cells proliferate under 80% O2. Under both 20% and 80% O2, mitochondrial reactive oxygen species production is 2fold less in HeLa-80 cells than in wild-type HeLa cells, related to a 2fold higher cytochrome-c oxidase activity. Elevated enzyme activity is associated with a 2fold increase in the regulatory subunit Vb. Treatment with siRNA against subunit Vb lowers its expression, increases mitochondrial generation of reactive oxygen species, decreases enzyme activity by 60-80% and diminishes viability under 80% O2 Homo sapiens
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HeLa-80 cell elevated COX activity in the oxygen-tolerant HeLa-80 cells, associated with a more than 2fold increase in the regulatory subunit COX Vb, whereas expression levels of other subunits are very close to wild-type HeLa-20 cells Homo sapiens
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additional information HeLa-20 cell Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + O2 + H+
-
 Homo sapiens ferricytochrome c + H2O
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 ?

Synonyms

Synonyms Comment Organism
COX Vb
-
 Homo sapiens
cytochrome c oxidase
-
 Homo sapiens