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Literature summary for 7.1.1.9 extracted from
- Purification and characterization of cytochrome c oxidase from Thermus thermophilus HB8 (1984), Biochemistry, 23, 454-460.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipid | activation | Thermus thermophilus |  |
| Triton X-100 | 0.5%, 10% activation | Thermus thermophilus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Thermus thermophilus | |
| Tween 20 | 0.5%, 40% inhibition | Thermus thermophilus | |
| Tween 80 | 0.5% 60% inhibition | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
ferrocytochrome c | Thermus thermophilus cytochrome c | Thermus thermophilus | |
| 0.041 | - |
ferrocytochrome c | horse cytochrome c | Thermus thermophilus | |
| 0.074 | - |
ferrocytochrome c | Candida krusei cytochrome c | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | - |
Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
| 71000 | - |
1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution into phospholipid vesicles, enzyme acts as proton pump | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 + H+ | horse cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r | |
| ferrocytochrome c + O2 + H+ | Thermus thermophilus cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r | |
| ferrocytochrome c + O2 + H+ | Candida krusei cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.88 | - |
ferrocytochrome c | horse cytochrome c | Thermus thermophilus | |
| 8.33 | - |
ferrocytochrome c | Candida krusei cytochrome c | Thermus thermophilus | |
| 53 | - |
ferrocytochrome c | Thermus thermophilus cytochrome c | Thermus thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme a | - |
Thermus thermophilus | |
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