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Literature summary for 7.1.1.9 extracted from
- Cytochrome c oxidase of Pseudomonas AM 1: purification, and molecular and enzymatic properties (1985), J. Biochem., 98, 493-499.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | 0.003 mM, 50% inhibition | Pseudomonas sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0087 | - |
ferrocytochrome c | Candida krusei cytochrome c | Pseudomonas sp. | |
| 0.05 | - |
ferrocytochrome c | horse and native cytochrome c | Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000 + 1 * 50000, SDS-PAGE | Pseudomonas sp. |
| 30000 | - |
x * 50000 + x * 30000, SDS-PAGE | Pseudomonas sp. |
| 50000 | - |
1 * 30000 + 1 * 50000, SDS-PAGE | Pseudomonas sp. |
| 50000 | - |
x * 50000 + x * 30000, SDS-PAGE | Pseudomonas sp. |
| 79000 | - |
SDS-PAGE, no mercaptoethanol | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
AM1 | - |
| Pseudomonas sp. AM1 | - |
AM1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, Sephacryl S-200 | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
cytochrome c | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c + O2 | horse ferrocytochrome c | Pseudomonas sp. | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Pseudomonas sp. | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | horse ferrocytochrome c | Pseudomonas sp. AM1 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Pseudomonas sp. AM1 | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 + H+ | - |
Pseudomonas sp. | ferricytochrome c + H2O | - |
r | |
| ferrocytochrome c + O2 + H+ | - |
Pseudomonas sp. AM1 | ferricytochrome c + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000 + x * 30000, SDS-PAGE | Pseudomonas sp. |
| dimer | 1 * 30000 + 1 * 50000, SDS-PAGE | Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 91 | - |
ferrocytochrome c | - |
Pseudomonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
electron donor: cytochrome c from Candida krusei | Pseudomonas sp. |
| 6.4 | - |
electron donor: cytochrome c from Pseudomonas sp. AM1 | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme a | - |
Pseudomonas sp. | |
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