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Literature summary for 7.1.1.8 extracted from

  • Guergova-Kuras, M.; Salcedo-Hernandez, R.; Bechmann, G.; Kuras, R.; Gennis, R.B.; Crofts, A.R.
    Expression and one-step purification of a fully active polyhistidine-tagged cytochrome bc1 complex from Rhodobacter sphaeroides (1999), Protein Expr. Purif., 15, 370-380.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fbcB and fbcC genes cloned using PCR amplification Cereibacter sphaeroides

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatophore
-
Cereibacter sphaeroides
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
19000
-
FeS-protein, SDS-PAGE Cereibacter sphaeroides
26000
-
gel filtration Cereibacter sphaeroides
32600
-
1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
34100
-
His-tagged cyt c1, SDS-PAGE Cereibacter sphaeroides
40100
-
1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
40800
-
His-tagged cyt b, SDS-PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
and strains BH6, CH6
-
Cereibacter sphaeroides BC17
-
and strains BH6, CH6
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-80°C, lyophilized in 50% v/v glycerol Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinol + 2 ferricytochrome c
-
Cereibacter sphaeroides ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
ubiquinol + 2 ferricytochrome c
-
Cereibacter sphaeroides BC17 ubiquinone + 2 ferrocytochrome c + 2 H+
-
?

Subunits

Subunits Comment Organism
dimer
-
Cereibacter sphaeroides
tetramer 1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
bc1 complex
-
Cereibacter sphaeroides