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Literature summary for 7.1.1.6 extracted from

  • Pierre, Y.; Breyton, C.; Kramer, D.; Popot, J.L.
    Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii (1995), J. Biol. Chem., 270, 29342-29349.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Stigmatellin complete inhibition at 0.001 mM Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
0°C, dark, 75% activity after 2 weeks Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plastoquinol + cytochrome f
-
Chlamydomonas reinhardtii plastoquinone + reduced cytochrome f
-
?
plastoquinol + plastocyanin
-
Chlamydomonas reinhardtii plastoquinone + reduced plastocyanin
-
?

Subunits

Subunits Comment Organism
dimer each subunit is present as 2 copies per b6/f dimer Chlamydomonas reinhardtii
More complex consists of polypeptides with the following molecular weights: cytochrome f MW 39500, cytochrome b6 MW 18000, Rieske FeS protein MW 18500, subunit IV MW 12500 and 3 proteins with a MW of 4000 each, SDS-PAGE Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.17 5 cytochrome f with plastocyanin as acceptor Chlamydomonas reinhardtii