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Literature summary for 7.1.1.6 extracted from
- A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts (1981), Eur. J. Biochem., 117, 591-599.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-dibromo-3-methyl-6-isopropylbenzoquinone | - |
Spinacia oleracea |  |
| 2-Iodo-6-isopropyl-3-methyl-2',2,4'-trinitrodiphenyl ether | - |
Spinacia oleracea | |
| bathophenanthroline | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| thylakoid | - |
Spinacia oleracea | 9579 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 2 nonheme irons per mol of cytochrome f | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
molecular mass added up after SDS-PAGE of subunits | Spinacia oleracea |
| 142000 | - |
calculation from cytochrome f content | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| five polypeptides | Spinacia oleracea |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution with lipids from spinach chloroplasts | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
14-20 micromol cytochrome c-552 reduced per nmol cytochrome f per h | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| plastoquinol + cytochrome f | - |
Spinacia oleracea | plastoquinone + reduced cytochrome f | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | complex consists of polypeptides with the following molecular weights: 34000, 33000, 23500, 20000, 17500, SDS-PAGE | Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | 34 | cytochrome f | - |
Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Spinacia oleracea |
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Release 2023.1 (January 2023)
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