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Literature summary for 7.1.1.6 extracted from

  • Clark, R.D.; Hind, G.
    Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts (1983), J. Biol. Chem., 258, 10348-10354.
    View publication on PubMed
Search for more literature for 7.1.1.6

General Stability

General Stability Organism
most stable in cholate buffer Spinacia oleracea
PMSF prevents proteolysis during purification Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxyquinoline N-oxide inhibition at low concentrations, 0.001-0.002 mM Spinacia oleracea
2-Iodo-6-isopropyl-3-methyl-2',2,4'-trinitrodiphenyl ether
-
 Spinacia oleracea
5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole inhibition at low concentrations, 0.001-0.002 mM Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 plastoquinol
-
 Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Storage Stability

Storage Stability Organism
0°C, 4 days months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plastoquinol + plastocyanin
-
 Spinacia oleracea plastoquinone + reduced plastocyanin
-
 ?

Subunits

Subunits Comment Organism
More complex consists of the following polypeptides: cytochrome f MW 33500 cytochrome b563 MW 22000, Rieske FeS protein MW 19000, subunit IV MW 16500, and a polypeptide with the molecular weight of 37000, SDS-PAGE Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
heme
-
 Spinacia oleracea