Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis strain LUW143 lacking both the aa3-600 menaquinol oxidase and caa3-type cytochrome c oxidase | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
menaquinol + O2 | Bacillus subtilis | cyt aa3-600 is strictly a menaquinol oxidase | menaquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, 1.25 equivalents of menaquinone-7 co-purify with the enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,3-dimethyl-1,4-naphthoquinol + O2 + n H+[side 1] | - |
Bacillus subtilis | 2 2,3-dimethyl-1,4-naphthoquinone + 2 H2O + n H+[side 2] | - |
? | |
menaquinol + O2 | cyt aa3-600 is strictly a menaquinol oxidase | Bacillus subtilis | menaquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyt aa3-600 | - |
Bacillus subtilis |
cytochrome aa3-600 menaquinol oxidase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
2,3-dimethyl-1,4-naphthoquinone | in 50 mM Tris, pH 7.0, at 25°C | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases | Bacillus subtilis |