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Literature summary for 7.1.1.5 extracted from

  • Yi, S.M.; Narasimhulu, K.V.; Samoilova, R.I.; Gennis, R.B.; Dikanov, S.A.
    Characterization of the semiquinone radical stabilized by the cytochrome aa3-600 menaquinol oxidase of Bacillus subtilis (2010), J. Biol. Chem., 285, 18241-18251.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis strain LUW143 lacking both the aa3-600 menaquinol oxidase and caa3-type cytochrome c oxidase Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
menaquinol + O2 Bacillus subtilis cyt aa3-600 is strictly a menaquinol oxidase menaquinone + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, 1.25 equivalents of menaquinone-7 co-purify with the enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,3-dimethyl-1,4-naphthoquinol + O2 + n H+[side 1]
-
Bacillus subtilis 2 2,3-dimethyl-1,4-naphthoquinone + 2 H2O + n H+[side 2]
-
?
menaquinol + O2 cyt aa3-600 is strictly a menaquinol oxidase Bacillus subtilis menaquinone + H2O
-
?

Synonyms

Synonyms Comment Organism
cyt aa3-600
-
Bacillus subtilis
cytochrome aa3-600 menaquinol oxidase
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
61
-
2,3-dimethyl-1,4-naphthoquinone in 50 mM Tris, pH 7.0, at 25°C Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
heme cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases Bacillus subtilis