Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.2 mM, complete inhibition | Sulfolobus acidocaldarius | |
additional information | no inhibition by myxothiazol, antimycin, funiculosin, 2-heptyl-4-hydroxyquinoline-N-oxide, 5-n-undecyl-6-hydroxy-2,7-dioxobenzothiazole and 3-(3,4-dichlorophenyl)-1,3-dimethylurea | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
N,N,N',N'-tetramethyl-1,4-phenylenediamine | pH 6. 40°C | Sulfolobus acidocaldarius | |
0.23 | - |
menadiol | pH 6. 40°C | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18900 | - |
x * 18900 (SoxA) + x * 58000 (SoxB) + x * 62600 (SoxC) + SoxD + ?. The SoxABCD quinol oxidase complex contains at least five different polypeptides. In addition to the major subunits SoxA, SoxB and SoxC, it has two small polypeptides. One of these is the translation product of a short open reading frame (called the soxD gene) at the end of the operon | Sulfolobus acidocaldarius |
58000 | - |
x * 18900 (SoxA) + x * 58000 (SoxB) + x * 62600 (SoxC) + SoxD + ?. The SoxABCD quinol oxidase complex contains at least five different polypeptides. In addition to the major subunits SoxA, SoxB and SoxC, it has two small polypeptides. One of these is the translation product of a short open reading frame (called the soxD gene) at the end of the operon | Sulfolobus acidocaldarius |
62600 | - |
x * 18900 (SoxA) + x * 58000 (SoxB) + x * 62600 (SoxC) + SoxD + ?. The SoxABCD quinol oxidase complex contains at least five different polypeptides. In addition to the major subunits SoxA, SoxB and SoxC, it has two small polypeptides. One of these is the translation product of a short open reading frame (called the soxD gene) at the end of the operon | Sulfolobus acidocaldarius |
280000 | - |
detergent-SoxABCD protein complex, gel filtration | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
caldariellaquinol + O2 + n H+/in | Sulfolobus acidocaldarius | the physiological electron donor of the SoxABCD complex is probably caldariellaquinol. Since caldariellaquinol is very hydrophobic and difficult to use in vitro, N,N,N',N'-tetramethyl-1,4-phenylenediamine is used as an artificial substrate | caldariellaquinone + H2O + n H+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.9 | - |
pH 6, 40°C, oxidation of N,N,N',N'-tetramethyl-1,4-phenylenediamine | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorobenzoquinol + oxidized dithiothreitol | - |
Sulfolobus acidocaldarius | ? | - |
? | |
caldariellaquinol + O2 + n H+/in | the physiological electron donor of the SoxABCD complex is probably caldariellaquinol. Since caldariellaquinol is very hydrophobic and difficult to use in vitro, N,N,N',N'-tetramethyl-1,4-phenylenediamine is used as an artificial substrate | Sulfolobus acidocaldarius | caldariellaquinone + H2O + n H+/out | - |
? | |
menadiol + oxidized dithiothreitol | - |
Sulfolobus acidocaldarius | menadione + dithiothreitol | - |
? | |
N,N,N',N'-tetramethyl-1,4-phenylenediamine + oxidized dithiothreitol | the physiological electron donor of the SoxABCD complex is probably caldariellaquinol. Since caldariellaquinol is very hydrophobic and difficult to use in vitro, N,N,N',N'-tetramethyl-1,4-phenylenediamine is used as a convenient artificial substrate | Sulfolobus acidocaldarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18900 (SoxA) + x * 58000 (SoxB) + x * 62600 (SoxC) + SoxD + ?. The SoxABCD quinol oxidase complex contains at least five different polypeptides. In addition to the major subunits SoxA, SoxB and SoxC, it has two small polypeptides. One of these is the translation product of a short open reading frame (called the soxD gene) at the end of the operon | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
SoxABCD quinol oxidase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Sulfolobus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | menadiol and 2,3,5,6-tetrachlorobenzoquinol show turnover numbers of 30-40 e-/s. The highest activity of 100 e-/s can be obtained with N,N,N',N'-tetramethyl-1,4-phenylenediamine | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the SoxABCD quinol oxidase complex probably contains four A-type hemes which are bound to SoxB and SoxC. The structure of these hemes is not identical to heme A. The Sulfolobus heme As has a 2-hydroxyethyl geranylgeranyl in position 2 of the porphyrin ring whereas heme A has the related farnesyl-containing side-chain | Sulfolobus acidocaldarius |