Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D75E | the mutant shows 48% activity with ubiquinol-1 and 88% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
F93A | the mutant shows 37% activity with ubiquinol-1 and 102% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
F93Y | the mutant shows 81% activity with ubiquinol-1 and 107% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
H98N | the mutant shows 3% activity with ubiquinol-1 and 10% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
H98T | the mutant shows 4% activity with ubiquinol-1 and 9% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
L160W | the mutant shows 58% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
N157V | the mutant shows 23% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101A | the mutant shows 26% activity with ubiquinol-1 and 82% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101E | the mutant shows 55% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101L | the mutant shows 11% activity with ubiquinol-1 and 58% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101M | the mutant shows 51% activity with ubiquinol-1 and 72% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101N | the mutant shows 10% activity with ubiquinol-1 and 61% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q101T | the mutant shows 27% activity with ubiquinol-1 and 62% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Q82A | the mutant shows 88% activity with ubiquinol-1 and no activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
R71Q | the mutant shows 3% activity with ubiquinol-1 and 8% activity with ubiquinol-2 compared to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-n-heptyl-4-hydroxyquinoline N-oxide | - |
Escherichia coli | |
aurachin C1-10 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
ubiquinol-1 | mutant enzyme D75E, at pH 7.0 and 25°C | Escherichia coli | |
0.024 | - |
ubiquinol-1 | mutant enzyme Q101M, at pH 7.0 and 25°C | Escherichia coli | |
0.03 | - |
ubiquinol-1 | mutant enzyme L160W, at pH 7.0 and 25°C | Escherichia coli | |
0.03 | - |
ubiquinol-1 | mutant enzyme Q101A, at pH 7.0 and 25°C | Escherichia coli | |
0.045 | - |
ubiquinol-1 | mutant enzyme Q82A, at pH 7.0 and 25°C | Escherichia coli | |
0.05 | - |
ubiquinol-1 | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
0.072 | - |
ubiquinol-1 | mutant enzyme F93Y, at pH 7.0 and 25°C | Escherichia coli | |
0.072 | - |
ubiquinol-1 | mutant enzyme Q101N, at pH 7.0 and 25°C | Escherichia coli | |
0.091 | - |
ubiquinol-1 | mutant enzyme F93A, at pH 7.0 and 25°C | Escherichia coli | |
0.1 | - |
ubiquinol-1 | mutant enzyme N157V, at pH 7.0 and 25°C | Escherichia coli | |
0.124 | - |
ubiquinol-1 | mutant enzyme Q101E, at pH 7.0 and 25°C | Escherichia coli | |
0.141 | - |
ubiquinol-1 | mutant enzyme Q101L, at pH 7.0 and 25°C | Escherichia coli | |
0.2 | - |
ubiquinol-1 | mutant enzyme Q101T, at pH 7.0 and 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | contains copper | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-8 + O2 + H+[side 1] | Escherichia coli | - |
ubiquinone-8 + H2O + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-1 + O2 + H+[side 1] | - |
Escherichia coli | ubiquinone-1 + H2O + H+[side 2] | - |
? | |
ubiquinol-2 + O2 + H+[side 1] | - |
Escherichia coli | ubiquinone-2 + H2O + H+[side 2] | - |
? | |
ubiquinol-8 + O2 + H+[side 1] | - |
Escherichia coli | ubiquinone-8 + H2O + H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome bo3 ubiquinol oxidase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
aurachin C1-10 | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
0.0007 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli |