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Literature summary for 7.1.1.3 extracted from

  • Li, M.; Khan, S.; Rong, H.; Tuma, R.; Hatzakis, N.S.; Jeuken, L.J.C.
    Effects of membrane curvature and pH on proton pumping activity of single cytochrome bo3 enzymes (2017), Biochim. Biophys. Acta, 1858, 763-770 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
E286C the mutant still reduces oxygen to oxidize ubiquinol, transporting chemical protons across the membrane in the process, but is unable to pump protons Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ubiquinol + O2 + H+[side 1] Escherichia coli
-
ubiquinone + H2O + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinol + O2 + H+[side 1]
-
Escherichia coli ubiquinone + H2O + H+[side 2]
-
?

Synonyms

Synonyms Comment Organism
cytochrome bo3
-
Escherichia coli